| dc.contributor.author |
Horst, MN |
en |
| dc.contributor.author |
Walker, AN |
en |
| dc.contributor.author |
Bush, P |
en |
| dc.contributor.author |
Wilson, T |
en |
| dc.contributor.author |
Chang, ES |
en |
| dc.contributor.author |
Miller, T |
en |
| dc.contributor.author |
Larkin, P |
en |
| dc.date.accessioned |
2014-06-06T06:47:26Z |
|
| dc.date.available |
2014-06-06T06:47:26Z |
|
| dc.date.issued |
2007 |
en |
| dc.identifier.issn |
1744117X |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.cbd.2006.11.004 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3591 |
|
| dc.subject |
Crustaceans |
en |
| dc.subject |
Decapods |
en |
| dc.subject |
Gene expression |
en |
| dc.subject |
Hepatopancreas |
en |
| dc.subject |
Insect growth regulators (IGR) |
en |
| dc.subject |
Lobsters |
en |
| dc.subject |
Methionine cycle |
en |
| dc.subject |
Methylation |
en |
| dc.subject |
Pesticides |
en |
| dc.subject |
Ubiquitination |
en |
| dc.subject.other |
aldehyde reductase |
en |
| dc.subject.other |
beta tubulin |
en |
| dc.subject.other |
betaine homocysteine methyltransferase |
en |
| dc.subject.other |
hemocyanin |
en |
| dc.subject.other |
initiation factor 2 |
en |
| dc.subject.other |
membrane protein |
en |
| dc.subject.other |
methionine |
en |
| dc.subject.other |
methoprene |
en |
| dc.subject.other |
pesticide |
en |
| dc.subject.other |
regulator protein |
en |
| dc.subject.other |
structural protein |
en |
| dc.subject.other |
trypsin |
en |
| dc.subject.other |
ubiquitin conjugating enzyme |
en |
| dc.subject.other |
ubiquitin thiolesterase |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
down regulation |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
gene identification |
en |
| dc.subject.other |
hepatopancreas |
en |
| dc.subject.other |
lobster |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein analysis |
en |
| dc.subject.other |
protein family |
en |
| dc.subject.other |
protein metabolism |
en |
| dc.subject.other |
quantitative analysis |
en |
| dc.subject.other |
real time polymerase chain reaction |
en |
| dc.subject.other |
subtractive hybridization |
en |
| dc.subject.other |
target cell |
en |
| dc.subject.other |
upregulation |
en |
| dc.subject.other |
Crustacea |
en |
| dc.subject.other |
Hexapoda |
en |
| dc.subject.other |
Homarus americanus |
en |
| dc.subject.other |
lobster |
en |
| dc.title |
Pesticide induced alterations in gene expression in the lobster, Homarus americanus |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.cbd.2006.11.004 |
en |
| heal.publicationDate |
2007 |
en |
| heal.abstract |
Using subtractive hybridization, we have identified 17 genes that are either up- or down-regulated in the hepatopancreas (Hp) of the lobster, Homarus americanus, by acute exposure to the juvenile hormone analog methoprene. The expression of some of the genes obtained from the subtraction libraries was confirmed by real time Q-PCR experiments. These genes encode several different classes of proteins including: structural, enzymatic and regulatory polypeptides. Enzymes represent the predominant genes up-regulated by methoprene. Included in this group are betaine-homocysteine S-methyltransferase (BHMT) and two other enzymes of the methionine cycle. Increased expression of a translation factor (eIF2), as well as of cytosolic (aldose reductase), structural (β-tubulin, L5A) and plasma membrane (CD42d) proteins was observed. In addition, a major feature of altered gene expression in methoprene treated Hp was increased levels of enzymes associated with protein turnover, including trypsin, ubiquitin conjugating enzyme and ubiquitin carboxyl terminal hydrolase. Down-regulation of the members of the hemocyanin family was observed. Assays confirmed elevated levels of trypsin in the Hp of lobsters after 24 h exposure to methoprene. Our findings suggest a wide variety of cellular targets are altered by methoprene. © 2006 Elsevier Inc. All rights reserved. |
en |
| heal.journalName |
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
2 |
en |
| dc.identifier.doi |
10.1016/j.cbd.2006.11.004 |
en |
| dc.identifier.spage |
44 |
en |
| dc.identifier.epage |
52 |
en |