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Effect of electrostatic spray parameters on the viability of two bacterial biocontrol agents and their deposition on blueberry flower stigmas

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dc.contributor.author Scherm, H en
dc.contributor.author Savelle, AT en
dc.contributor.author Law, SE en
dc.date.accessioned 2014-06-06T06:47:22Z
dc.date.available 2014-06-06T06:47:22Z
dc.date.issued 2007 en
dc.identifier.issn 09583157 en
dc.identifier.uri http://dx.doi.org/10.1080/09583150701211616 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3549
dc.subject Bacillus subtilis en
dc.subject Biological control en
dc.subject Blueberry en
dc.subject Charged droplet en
dc.subject Electrostatic spray en
dc.subject Pseudomonas fluorescens en
dc.subject.other bacterium en
dc.subject.other biocontrol agent en
dc.subject.other biopesticide en
dc.subject.other fungal disease en
dc.subject.other fungicide en
dc.subject.other fungus en
dc.subject.other shrub en
dc.subject.other Bacillus subtilis en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Fungi en
dc.subject.other Monilinia vaccinii-corymbosi en
dc.subject.other Pseudomonas fluorescens en
dc.subject.other Vaccinium en
dc.title Effect of electrostatic spray parameters on the viability of two bacterial biocontrol agents and their deposition on blueberry flower stigmas en
heal.type journalArticle en
heal.identifier.primary 10.1080/09583150701211616 en
heal.publicationDate 2007 en
heal.abstract The fungus Monilinia vaccinii-corymbosi infects blueberry flowers via the stigma-style ovary pathway to cause mummy berry disease. Previous laboratory experiments documented considerable activity of stigma-applied biofungicides containing the bacteria Bacillus subtilis and, to a lesser extent, Pseudomonas fluorescens against flower infection by the pathogen. However, adequate and targeted delivery of the biocontrol agents to the stigmatic surfaces of open flowers in the field has remained problematic. Here we consider the application of the biofungicides Serenade AS (containing B. subtilis QST713) and BlightBan A506 (containing P. fluorescens A506) to blueberry flowers by air-assisted electrostatic spraying. In laboratory experiments with typical field-use rates, viability of B. subtilis and P. fluorescens was unaffected by different levels of induction-charging voltage (0-1.2 kV) and atomizing pressure (138-276 kPa) applied to an electrostatic spray-charging nozzle, showing that the bacteria in both formulations readily survived exposure to the intense electrical fields and near-sonic atomizing air shear encountered during electrostatic spraying. Electrostatically charged application significantly (P≤0.0001) increased deposition of B. subtilis on the stigmatic surfaces of detached blueberry flower clusters by a factor of 4.5 compared with conventional hydraulic spraying; a similar comparison showed that population densities of P. fluorescens on the stigma were increased by a factor of 2.9, but this effect was not statistically significant (P=0.1487). For Serenade, the increased coverage and/or retention on the flower stigma, along with the excellent bacterial survival, portend well for electrostatic application for mummy berry disease control in the field. © 2007 Taylor & Francis. en
heal.journalName Biocontrol Science and Technology en
dc.identifier.issue 3 en
dc.identifier.volume 17 en
dc.identifier.doi 10.1080/09583150701211616 en
dc.identifier.spage 285 en
dc.identifier.epage 293 en


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