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Trichome layers versus dehaired lamina of Olea europaea leaves: Differences in flavonoid distribution, UV-absorbing capacity, and wax yield

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dc.contributor.author Liakopoulos, G en
dc.contributor.author Stavrianakou, S en
dc.contributor.author Karabourniotis, G en
dc.date.accessioned 2014-06-06T06:47:16Z
dc.date.available 2014-06-06T06:47:16Z
dc.date.issued 2006 en
dc.identifier.issn 00988472 en
dc.identifier.uri http://dx.doi.org/10.1016/j.envexpbot.2004.11.008 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3503
dc.subject Cuticular waxes en
dc.subject Flavonoid tissue distribution en
dc.subject Leaf pubescence en
dc.subject Olea europaea en
dc.subject Trichome en
dc.subject UV-absorbing capacity en
dc.subject.other leaf en
dc.subject.other secondary metabolite en
dc.subject.other trichome en
dc.subject.other Olea europaea en
dc.title Trichome layers versus dehaired lamina of Olea europaea leaves: Differences in flavonoid distribution, UV-absorbing capacity, and wax yield en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.envexpbot.2004.11.008 en
heal.publicationDate 2006 en
heal.abstract Leaf flavonoid compounds from six olive (Olea europaea L.) cultivars were analysed by HPLC. The composition of the soluble fractions of the dehaired lamina and the isolated trichome layers of the abaxial leaf surface were analysed in separate, as to study the distribution of flavonoids between the two leaf parts. Quercetin and quercetin 3-O-rhamnoside that have been reported to occur in the leaves seem to be located exclusively in the trichome layer. A greater variety of flavonoids was found in the lamina but the trichome layer was richer in terms of total flavonoids per unit mass. Trichome layer demonstrated an independent chemical character since its flavonoid concentration and composition was not coordinated with that of the lamina. The occurrence of flavonoid compounds in trichomes is related to the UV-filtering capacity of these cells. The results of the present study showed that apart from the soluble fraction, the cuticular waxes and cell walls of the trichomes also showed significant UV-absorbing capacity, indicating the occurrence of UV-absorbing compounds in these fractions as well. Moreover, the cuticular waxes of the trichome layer exhibited not only a higher investment of mass per unit of leaf area, but also a higher UV-absorbing capacity expressed per unit mass as compared to the cuticular waxes of the lamina surface. The importance for the separate chemical investigation of the phenolic composition of the leaf lamina and the trichome layers as well as the ecological significance of the findings is discussed. © 2004 Elsevier B.V. All rights reserved. en
heal.journalName Environmental and Experimental Botany en
dc.identifier.issue 3 en
dc.identifier.volume 55 en
dc.identifier.doi 10.1016/j.envexpbot.2004.11.008 en
dc.identifier.spage 294 en
dc.identifier.epage 304 en


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