The influence of gelling agent purity and ion additions on in vitro tomato pollen germinability and pollen tube growth on semi-solid substrates

dc.contributor.author	Karapanos, IC	en
dc.contributor.author	Akoumianakis, K	en
dc.contributor.author	Olympios, CM	en
dc.contributor.author	Passam, HC	en
dc.date.accessioned	2014-06-06T06:47:16Z
dc.date.available	2014-06-06T06:47:16Z
dc.date.issued	2006	en
dc.identifier.issn	01676857	en
dc.identifier.uri	http://dx.doi.org/10.1007/s11240-006-9152-9	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3494
dc.subject	Agar	en
dc.subject	Agarose	en
dc.subject	Ion content	en
dc.subject	Lycopersicon esculentum	en
dc.subject	Washed agar	en
dc.subject.other	Sodium	en
dc.subject.other	Substrates	en
dc.subject.other	Agarose	en
dc.subject.other	Ion content	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Semi solid substrates	en
dc.subject.other	Washed agar	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Agar	en
dc.subject.other	Germination	en
dc.subject.other	Growth	en
dc.subject.other	Kinetics	en
dc.subject.other	Pollen	en
dc.subject.other	Sodium	en
dc.subject.other	Substrates	en
dc.subject.other	Lycopersicon esculentum	en
dc.title	The influence of gelling agent purity and ion additions on in vitro tomato pollen germinability and pollen tube growth on semi-solid substrates	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11240-006-9152-9	en
heal.publicationDate	2006	en
heal.abstract	The physicochemical properties and inherent ion content of the gelling agents used for the preparation of semi-solid substrates significantly affect the germination of tomato pollen in vitro. The addition of Ca, K and Mg to semi-solid, agar-based, substrates improved the germination of tomato pollen when the inherent Ca content of the agar was low, but was without effect or even inhibitory when the Ca level was high. However, Κ and/or Mg addition was beneficial irrespective of the agar source. When agarose replaced agar and K, Mg and Ca were added individually or in combination, pollen germination and pollen tube growth were affected differently by each ion but were optimal only in the presence of all three ions, reflecting the absence of these ions in agarose. An involvement of Na was also implicated since reduction of the inherently high Na content of agar by washing improved germination, with or without the addition of Κ, Mg and Ca. Since >3 mM Ca in the semi-solid substrate impairs tomato pollen germination, the gelling agent must be of high purity, which in the case of agar may entail washing, followed by the addition of K, Mg and Ca. The adoption of such a medium would permit the standardization of semi-solid substrates for in vitro tomato pollen germination studies. © 2006 Springer Science+Business Media B.V.	en
heal.journalName	Plant Cell, Tissue and Organ Culture	en
dc.identifier.issue	2	en
dc.identifier.volume	87	en
dc.identifier.doi	10.1007/s11240-006-9152-9	en
dc.identifier.spage	181	en
dc.identifier.epage	190	en