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Molecular cloning and characterization of Gossypium hirsutum superoxide dismutase genes during cotton-Xanthomonas campestris pv. malvacearum interaction

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dc.contributor.author Voloudakis, AE en
dc.contributor.author Marmey, P en
dc.contributor.author Delannoy, E en
dc.contributor.author Jalloul, A en
dc.contributor.author Martinez, C en
dc.contributor.author Nicole, M en
dc.date.accessioned 2014-06-06T06:47:10Z
dc.date.available 2014-06-06T06:47:10Z
dc.date.issued 2006 en
dc.identifier.issn 08855765 en
dc.identifier.uri http://dx.doi.org/10.1016/j.pmpp.2006.09.001 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3427
dc.subject Cotton (Gossypium hirsutum) en
dc.subject Hypersensitive reaction en
dc.subject Oxidative stress en
dc.subject SOD en
dc.subject Superoxide dismutase gene en
dc.subject.other Gossypium hirsutum en
dc.subject.other Xanthomonas campestris en
dc.subject.other Xanthomonas campestris pv. malvacearum en
dc.title Molecular cloning and characterization of Gossypium hirsutum superoxide dismutase genes during cotton-Xanthomonas campestris pv. malvacearum interaction en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.pmpp.2006.09.001 en
heal.publicationDate 2006 en
heal.abstract Total superoxide dismutase (SOD) enzymatic activity of cotton (Gossypium hirsutum L.) cotyledons peaked 4 h post-infiltration (hpi) with the avirulent Xanthomonas campestris pv. malvacearum race 18 (Xcm18), contributing to the oxidative burst observed in the hypersensitive reaction (HR). cDNA fragments encoding cotton SOD genes were isolated by RT-PCR, sequenced and their similarities to known Cu/ZnSOD (cytoplasmic and chloroplastic), MnSOD and FeSOD were established based on amino acid sequences. Transcriptional expression of cotton SODs, during the development of cotton HR, was studied using a semiquantitative RT-PCR analysis. Cu/ZnSODcyt and Cu/ZnSODchl transcripts were similar in both the compatible and incompatible interactions. However, in the incompatible interaction MnSOD was induced 2 hpi and remained at higher levels when compared to the compatible interaction, and FeSOD was induced 6 hpi and peaked at 10-12 hpi. These results suggest that MnSOD may contribute to the SOD activity observed 4 hpi playing a role in the early apoptotic events of the HR in cotton-Xcm interaction. Cotton FeSOD gene expression pattern postulates a contribution of this enzyme in the later stages of HR development. Furthermore, catalase 1 was transcriptionally stable in cotton-Xcm interaction, with a slightly increased level in the incompatible interaction. © 2006. en
heal.journalName Physiological and Molecular Plant Pathology en
dc.identifier.issue 4-6 en
dc.identifier.volume 68 en
dc.identifier.doi 10.1016/j.pmpp.2006.09.001 en
dc.identifier.spage 119 en
dc.identifier.epage 127 en


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