Low-boron acclimation induces uptake of boric acid against a concentration gradient in root cells of Olea europaea

dc.contributor.author	Stavrianakou, S	en
dc.contributor.author	Liakopoulos, G	en
dc.contributor.author	Karvonis, E	en
dc.contributor.author	Resta, E	en
dc.contributor.author	Karabourniotis, G	en
dc.date.accessioned	2014-06-06T06:47:09Z
dc.date.available	2014-06-06T06:47:09Z
dc.date.issued	2006	en
dc.identifier.issn	14454408	en
dc.identifier.uri	http://dx.doi.org/10.1071/FP05097	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3421
dc.subject	Chromotropic acid	en
dc.subject	HPLC assay	en
dc.subject	Olive tree	en
dc.subject	Root cell sap	en
dc.subject	Xylem exudates	en
dc.subject.other	Bioassay	en
dc.subject.other	Boron compounds	en
dc.subject.other	Cells	en
dc.subject.other	Diffusion	en
dc.subject.other	Chromotropic acids	en
dc.subject.other	HPLC assays	en
dc.subject.other	Olive trees	en
dc.subject.other	Root cell sap	en
dc.subject.other	Xylem exudates	en
dc.subject.other	Plants (botany)	en
dc.subject.other	boron	en
dc.subject.other	Biological Tests	en
dc.subject.other	Boron Compounds	en
dc.subject.other	Cells	en
dc.subject.other	Diffusion	en
dc.subject.other	Plants	en
dc.subject.other	Olea europaea	en
dc.title	Low-boron acclimation induces uptake of boric acid against a concentration gradient in root cells of Olea europaea	en
heal.type	journalArticle	en
heal.identifier.primary	10.1071/FP05097	en
heal.publicationDate	2006	en
heal.abstract	Low concentrations of boron (B) in the external medium can induce uptake mechanisms whereby plants can develop a concentration gradient for B against the external medium. These mechanisms seem to be widespread among herbaceous species. In this study, olive (Olea europaea L.) plants were acclimated to either high (23 μM; controls) or low (0.5 μM; low-B plants) concentrations of B for 45 d, in a hydroponic culture. Afterwards, a 7-h uptake experiment was conducted by transferring plants of both groups to a series of nutrient solutions with B concentrations ranging from 0.5 to 23 μM. Analysis of B concentration in cell sap of root and xylem exudate was performed by the borate-chromotropic acid HPLC assay. Plants acclimated to high-B concentration showed root cell and xylem exudate B concentrations that were comparable to those of the external medium. In contrast, plants acclimated to low-B concentration were able to develop concentrations of B in root cells up to 2-fold higher than those of the external medium. Moreover, B concentrations in xylem exudate for both plant groups corresponded to those of the root cell sap, indicating diffusion equilibrium. These results support the existence of a mechanism that concentrates B in the root cell sap against the nutrient solution when olive plants are acclimated to low-B conditions. © CSIRO 2006.	en
heal.journalName	Functional Plant Biology	en
dc.identifier.issue	2	en
dc.identifier.volume	33	en
dc.identifier.doi	10.1071/FP05097	en
dc.identifier.spage	189	en
dc.identifier.epage	193	en