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Kenaf xylan - A source of biologically active acidic oligosaccharides

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dc.contributor.author Nacos, MK en
dc.contributor.author Katapodis, P en
dc.contributor.author Pappas, C en
dc.contributor.author Daferera, D en
dc.contributor.author Tarantilis, PA en
dc.contributor.author Christakopoulos, P en
dc.contributor.author Polissiou, M en
dc.date.accessioned 2014-06-06T06:47:09Z
dc.date.available 2014-06-06T06:47:09Z
dc.date.issued 2006 en
dc.identifier.issn 01448617 en
dc.identifier.uri http://dx.doi.org/10.1016/j.carbpol.2006.02.032 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3415
dc.subject 13C NMR spectroscopy en
dc.subject Endoxylanase en
dc.subject Extraction en
dc.subject FT-IR spectroscopy en
dc.subject Hemicellulose en
dc.subject Kenaf en
dc.subject Oligosaccharide en
dc.subject Uronic acid en
dc.subject Xylan en
dc.subject.other Cellulose en
dc.subject.other Enzyme kinetics en
dc.subject.other Fourier transform infrared spectroscopy en
dc.subject.other Hydrolysis en
dc.subject.other Nuclear magnetic resonance spectroscopy en
dc.subject.other Aldouronic acids en
dc.subject.other Endoxylanase en
dc.subject.other Hemicellulose en
dc.subject.other Oligomers en
dc.subject.other Infrared Spectra en
dc.subject.other Kenaf en
dc.subject.other Oligosaccharides en
dc.subject.other Uronic Acids en
dc.subject.other Xylans en
dc.subject.other Corynascus heterothallicus en
dc.subject.other Hibiscus en
dc.subject.other Hibiscus cannabinus en
dc.subject.other Sporothrix en
dc.subject.other Thermoascus en
dc.subject.other Thermoascus aurantiacus en
dc.title Kenaf xylan - A source of biologically active acidic oligosaccharides en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.carbpol.2006.02.032 en
heal.publicationDate 2006 en
heal.abstract Two xylose-rich hemicellulose fractions were obtained from kenaf wood (Hibiscus cannabinus L.) through a series of sequential extractions which dissociated xylans from other cell wall components. These fractions were subsequently used as substrates for the production of biologically active aldouronic acids. Incubation of the xylans with a family 10 Thermoascus aurantiacus endoxylanase resulted in the isolation of an aldotetrauronic acid as the main acidic oligosaccharide in the hydrolysis products. Enzymic hydrolysis with a family 11 Sporotrichum thermophile endoxylanase instead resulted in the isolation of a aldopentauronic acid as the main acidic oligosaccharide. The identity and purity of both xylans and aldouronic acids were assessed with solid-state FT-IR, solid-state and solution 13C NMR spectroscopy. © 2006 Elsevier Ltd. All rights reserved. en
heal.journalName Carbohydrate Polymers en
dc.identifier.issue 1 en
dc.identifier.volume 66 en
dc.identifier.doi 10.1016/j.carbpol.2006.02.032 en
dc.identifier.spage 126 en
dc.identifier.epage 134 en


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