Isolation and characterization of stress related Heat shock protein calmodulin binding gene from cultivated cotton (Gossypium hirsutum L.)

dc.contributor.author	Sotirios, KA	en
dc.contributor.author	Argyrokastritis, A	en
dc.contributor.author	Loukas, M	en
dc.contributor.author	Eliopoulos, E	en
dc.contributor.author	Tsakas, S	en
dc.contributor.author	Kaltsikes, PJ	en
dc.date.accessioned	2014-06-06T06:47:08Z
dc.date.available	2014-06-06T06:47:08Z
dc.date.issued	2006	en
dc.identifier.issn	00142336	en
dc.identifier.uri	http://dx.doi.org/10.1007/s10681-005-9028-9	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3414
dc.subject	Genomic organization	en
dc.subject	Gossypium hirsutum L.	en
dc.subject	Heat-shock protein calmodulin binding gene	en
dc.subject	Osmotic stress	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Gossypium hirsutum	en
dc.subject.other	Nicotiana tabacum	en
dc.subject.other	Penicillium	en
dc.subject.other	Penicillium camemberti	en
dc.subject.other	Rhizomucor	en
dc.subject.other	Rhizomucor miehei	en
dc.subject.other	Rhizopus oryzae	en
dc.subject.other	Thermomyces lanuginosus	en
dc.title	Isolation and characterization of stress related Heat shock protein calmodulin binding gene from cultivated cotton (Gossypium hirsutum L.)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10681-005-9028-9	en
heal.publicationDate	2006	en
heal.abstract	Due to the important role of cotton drought tolerant varieties and the reported involvement in this trait of Heat-shock protein calmodulin binding, the respective gene (HSPCB) was isolated and characterized from cultivated cotton, Gossypium hirsutum (Zeta 2 cultivar), using a chromosome-walking technique. Segments of 3079 bp genomic and 1572 bp cDNA sequences were obtained. Alignments revealed that the HSPCB cDNA contains a single open reading frame of 1374 bp comprising the complete coding region and that the gene possesses five exons. The predicted amino acid sequence shares 72, 54, 58, 32, 31, 27, 28 and 27% identities with A. thaliana, rice, tobacco, Penicillium camemberti, Rhizopus oryzae, Thermomyces lanuginosus, Rhizomucor miehei, Aspergillus niger Lipase/HSPCB proteins, respectively. In addition, the expression profile of this gene was preliminary studied in different plant tissues. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using total RNA samples from leaves, stems and roots of the Australian cotton variety Siokra L23, known for its drought tolerance, isolated after a 4-d osmotic stress treatment (i.e. at the end of the stress period). Semi-quantitative transcription analysis indicated that, under the experimental conditions employed in present study, the gene is expressed in all plant tissues examined. © Springer 2006.	en
heal.journalName	Euphytica	en
dc.identifier.issue	3	en
dc.identifier.volume	147	en
dc.identifier.doi	10.1007/s10681-005-9028-9	en
dc.identifier.spage	343	en
dc.identifier.epage	351	en