Inhibitory activity on amyloid-β aggregation and antioxidant properties of Crocus sativus stigmas extract and its crocin constituents

dc.contributor.author	Papandreou, MA	en
dc.contributor.author	Kanakis, CD	en
dc.contributor.author	Polissiou, MG	en
dc.contributor.author	Efthimiopoulos, S	en
dc.contributor.author	Cordopatis, P	en
dc.contributor.author	Margarity, M	en
dc.contributor.author	Lamari, FN	en
dc.date.accessioned	2014-06-06T06:47:08Z
dc.date.available	2014-06-06T06:47:08Z
dc.date.issued	2006	en
dc.identifier.issn	00218561	en
dc.identifier.uri	http://dx.doi.org/10.1021/jf061932a	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3407
dc.subject	Alzheimer's disease	en
dc.subject	Antioxidant	en
dc.subject	Crocin	en
dc.subject	Crocus sativus	en
dc.subject	Fibrillogenesis	en
dc.subject	Stigmas	en
dc.subject.other	amyloid beta protein	en
dc.subject.other	amyloid beta protein[1-40]	en
dc.subject.other	antioxidant	en
dc.subject.other	carotenoid	en
dc.subject.other	crocin	en
dc.subject.other	peptide fragment	en
dc.subject.other	plant extract	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	Crocus	en
dc.subject.other	drug antagonism	en
dc.subject.other	flower	en
dc.subject.other	metabolism	en
dc.subject.other	Amyloid beta-Protein	en
dc.subject.other	Antioxidants	en
dc.subject.other	Carotenoids	en
dc.subject.other	Crocus	en
dc.subject.other	Flowers	en
dc.subject.other	Peptide Fragments	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Crocus	en
dc.subject.other	Crocus sativus	en
dc.subject.other	Daucus carota	en
dc.subject.other	Lycopersicon esculentum	en
dc.title	Inhibitory activity on amyloid-β aggregation and antioxidant properties of Crocus sativus stigmas extract and its crocin constituents	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/jf061932a	en
heal.publicationDate	2006	en
heal.abstract	Crocus sativus stigmas are one of the widely known spices (saffron) and consist of unusually polar carotenoids. Alzheimer's disease is characterized pathologically by deposition of amyloid β-peptide (Aβ) fibrils. Oxidation is thought to promote Aβ fibril formation and deposition. To identify agents inhibiting the pathogenesis of Alzheimer's disease, we examined in vitro the antioxidant properties of extract of C. sativus stigmas and its effect on Aβ 1-40 fibrillogenesis. The antioxidant properties were determined by measuring the ferric-reducing antioxidant power and Trolox-equivalent antioxidant capacity, while its effects on Aβ-aggregation and fibrillogenesis were studied by thioflavine T-based fluorescence assay and by DNA binding shift assay. The water:methanol (50:50, v/v) extract of C. sativus stigmas possesses good antioxidant properties, higher than those of tomatoes and carrots, and inhibited Aβ fibrillogenesis in a concentration and time-dependent manner. The main carotenoid constituent, trans-crocin-4, the digentibiosyl ester of crocetin, inhibited Aβ fibrillogenesis at lower concentrations than dimethylcrocetin, revealing that the action of the carotenoid is enhanced by the presence of the sugars. Our findings suggest the possible use of C. sativus stigma constituents for inhibition of aggregation and deposition of Aβ in the human brain. © 2006 American Chemical Society.	en
heal.journalName	Journal of Agricultural and Food Chemistry	en
dc.identifier.issue	23	en
dc.identifier.volume	54	en
dc.identifier.doi	10.1021/jf061932a	en
dc.identifier.spage	8762	en
dc.identifier.epage	8768	en