Influence of glucose and saturated free-fatty acid mixtures on citric acid and lipid production by Yarrowia lipolytica

dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Chevalot, I	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Marc, I	en
dc.contributor.author	Aggelis, G	en
dc.date.accessioned	2014-06-06T06:47:07Z
dc.date.available	2014-06-06T06:47:07Z
dc.date.issued	2006	en
dc.identifier.issn	03438651	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00284-005-0223-7	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3402
dc.subject.other	citric acid	en
dc.subject.other	fatty acid	en
dc.subject.other	glucose	en
dc.subject.other	lipid	en
dc.subject.other	nitrogen	en
dc.subject.other	tristearin	en
dc.subject.other	animal cell	en
dc.subject.other	article	en
dc.subject.other	catabolism	en
dc.subject.other	cell culture	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	measurement	en
dc.subject.other	nonhuman	en
dc.subject.other	parameter	en
dc.subject.other	prediction	en
dc.subject.other	priority journal	en
dc.subject.other	quantitative analysis	en
dc.subject.other	sugar intake	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	yeast	en
dc.subject.other	yeast cell	en
dc.subject.other	Citric Acid	en
dc.subject.other	Culture Media	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Glucose	en
dc.subject.other	Lipids	en
dc.subject.other	Yarrowia	en
dc.subject.other	Animalia	en
dc.subject.other	Yarrowia lipolytica	en
dc.title	Influence of glucose and saturated free-fatty acid mixtures on citric acid and lipid production by Yarrowia lipolytica	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00284-005-0223-7	en
heal.publicationDate	2006	en
heal.abstract	In the present report, the effect of glucose and stearin (substrate composed by saturated free-fatty acids) on the production of biomass, reserve lipid, and citric acid by Yarrowia lipolytica ACA-DC 50109 was investigated in nitrogen-limited cultures. Numerical models that were used in order to quantify the kinetic behavior of the above Yarrowia lipolytica strain showed successful simulation, while the optimized parameter values were similar to those experimentally measured and the predictive ability of the models was satisfactory. In nitrogen-limited cultures in which glucose was used as the sole substrate, satisfactory growth and no glucose inhibition occurred, although in some cases the initial concentration of glucose was significantly high (150 g/l). Citric acid production was observed in all trials, which was in some cases notable (final concentration 42.9 g/l, yield 0.56 g per g of sugar consumed). The concentration of unsaturated cellular fatty acids was slightly lower when the quantity of sugar in the medium was elevated. In the cases in which stearin and glucose were used as co-substrates, in spite of the fact that the quantity of cellular lipid inside the yeast cells varied remarkably (from 0.3 to 2.0 g/l - 4 to 20% wt/wt), de novo fatty acid biosynthesis was observed. This activity increased when the yeast cells assimilated higher sugar quantities. The citric acid produced was mainly derived from the catabolism of sugar. Nevertheless, citric acid yield on sugar consumed and citrate specific production rate, as evaluated by the numerical model, presented substantially higher values in the fermentation in which no fat was used as glucose co-substrate compared with the cultures with stearin used as co-substrate. © Springer Science+Business Media, Inc. 2006.	en
heal.journalName	Current Microbiology	en
dc.identifier.issue	2	en
dc.identifier.volume	52	en
dc.identifier.doi	10.1007/s00284-005-0223-7	en
dc.identifier.spage	134	en
dc.identifier.epage	142	en