Effect of the combined use of Metarhizium anisopliae (Metschinkoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species

dc.contributor.author	Kavallieratos, NG	en
dc.contributor.author	Athanassiou, CG	en
dc.contributor.author	Michalaki, MP	en
dc.contributor.author	Batta, YA	en
dc.contributor.author	Rigatos, HA	en
dc.contributor.author	Pashalidou, FG	en
dc.contributor.author	Balotis, GN	en
dc.contributor.author	Tomanovic, Z	en
dc.contributor.author	Vayias, BJ	en
dc.date.accessioned	2014-06-06T06:47:05Z
dc.date.available	2014-06-06T06:47:05Z
dc.date.issued	2006	en
dc.identifier.issn	02612194	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.cropro.2006.02.009	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3377
dc.subject	Diatomaceous earth	en
dc.subject	Metarhizium anisopliae	en
dc.subject	Rhyzopertha dominica	en
dc.subject	Sitophilus oryzae	en
dc.subject	Tribolium confusum	en
dc.subject.other	beetle	en
dc.subject.other	bioassay	en
dc.subject.other	biocontrol agent	en
dc.subject.other	biological control	en
dc.subject.other	crop pest	en
dc.subject.other	diatomite	en
dc.subject.other	fungus	en
dc.subject.other	laboratory method	en
dc.subject.other	mortality	en
dc.subject.other	wheat	en
dc.subject.other	Bostrichidae	en
dc.subject.other	Coleoptera	en
dc.subject.other	Curculionidae	en
dc.subject.other	Fungi	en
dc.subject.other	Fungi imperfecti	en
dc.subject.other	Hexapoda	en
dc.subject.other	Hyphomycetes	en
dc.subject.other	Metarhizium anisopliae	en
dc.subject.other	Oryzaephilus	en
dc.subject.other	Rhyzopertha dominica	en
dc.subject.other	Sitophilus oryzae	en
dc.subject.other	Tenebrionidae	en
dc.subject.other	Tribolium (beetle)	en
dc.subject.other	Tribolium confusum	en
dc.subject.other	Triticum aestivum	en
dc.title	Effect of the combined use of Metarhizium anisopliae (Metschinkoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.cropro.2006.02.009	en
heal.publicationDate	2006	en
heal.abstract	Laboratory bioassays were conducted in order to evaluate the use of the entomopathogenic fungus, Metarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes), against adults of three stored-grain beetle species, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae). Two fungal preparations were compared, a conidial suspension and a conidial powder. These were applied to wheat, at three dosages, 8×106, 8×108 and 8×1010 conidia/kg of wheat, alone, or in combination with the diatomaceous earth (DE) formulation SilicoSec® (Biofa Gbmh, Germany), applied at 0.5 g/kg of wheat. Adult insects were exposed to treated wheat for 24 h, 48 h, 7 d and 14 d. The mortality of R. dominica adults after 14 d of exposure to the treated substrate was 100% and 96% at the dosages in combination, for the suspension and the powder, respectively. The respective figures without DE were 94.4% and 74.6%. In contrast, against S. oryzae adults, the application of conidial suspension combined with DE was not as effective as the application of DE alone. Adult mortality of S. oryzae increased notably on wheat treated with the conidial powder. Similarly, the conidial suspension, with or without DE, was not as effective against T. confusum as the conidial powder. The progeny production of R. dominica on wheat treated with the highest suspension dosage, with or without DE, was significantly lower than that for the other aqueous fungal dosages. Moreover, significantly less progeny were produced on wheat treated with the highest dosage of powder conidia combined with DE, in comparison with the other treatments. In contrast, S. oryzae progeny production was notably reduced only in wheat treated with the highest dosage of fungal spore powder mixed with DE. No T. confusum progeny were found on wheat treated with the highest dosage of both fungal preparations combined with DE. © 2006 Elsevier Ltd. All rights reserved.	en
heal.journalName	Crop Protection	en
dc.identifier.issue	10	en
dc.identifier.volume	25	en
dc.identifier.doi	10.1016/j.cropro.2006.02.009	en
dc.identifier.spage	1087	en
dc.identifier.epage	1094	en