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Effect of nisin on growth boundaries of Listeria monocytogenes Scott A, at various temperatures, pH and water activities

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dc.contributor.author Boziaris, IS en
dc.contributor.author Nychas, G-JE en
dc.date.accessioned 2014-06-06T06:47:04Z
dc.date.available 2014-06-06T06:47:04Z
dc.date.issued 2006 en
dc.identifier.issn 07400020 en
dc.identifier.uri http://dx.doi.org/10.1016/j.fm.2006.03.003 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3374
dc.subject Growth/no growth en
dc.subject Listeria en
dc.subject Nisin en
dc.subject.other antiinfective agent en
dc.subject.other nisin en
dc.subject.other water en
dc.subject.other article en
dc.subject.other bacterial count en
dc.subject.other biological model en
dc.subject.other drug effect en
dc.subject.other food control en
dc.subject.other food preservation en
dc.subject.other growth, development and aging en
dc.subject.other kinetics en
dc.subject.other Listeria monocytogenes en
dc.subject.other metabolism en
dc.subject.other methodology en
dc.subject.other pH en
dc.subject.other statistical model en
dc.subject.other temperature en
dc.subject.other Anti-Bacterial Agents en
dc.subject.other Colony Count, Microbial en
dc.subject.other Food Microbiology en
dc.subject.other Food Preservation en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Kinetics en
dc.subject.other Listeria monocytogenes en
dc.subject.other Logistic Models en
dc.subject.other Models, Biological en
dc.subject.other Nisin en
dc.subject.other Temperature en
dc.subject.other Water en
dc.subject.other Listeria monocytogenes en
dc.title Effect of nisin on growth boundaries of Listeria monocytogenes Scott A, at various temperatures, pH and water activities en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.fm.2006.03.003 en
heal.publicationDate 2006 en
heal.abstract The effect of nisin on growth boundaries of Listeria monocytogenes Scott A in Tryptone Soy Broth (TSB) under different aws, pH, and temperatures was studied. Growth/no growth turbidity data was modeled using logistic regression. Combinations of various temperatures (5-35 °C), pH (4.05-6.70) adjusted with HCl, aws (0.937-0.998) NaCl (0.5-10.5%) and nisin (0-100 IU/ml) were used to monitor the growth/no growth response of L. monocytogenes Scott A for 60 days. The concordance of the logistic regression model was 99.4%, indicating successful data fitting. The minimum pH at which growth was observed was 4.81 at the temperature range of 25-35 °C and at aw as high as 0.992. Growth was observed at aw as low as 0.937, at pH 6.7, at the temperature range of 25-35 °C. Increasing nisin concentrations above 25 IU/ml resulted in a more inhibitory environment for L. monocytogenes. Presence of 100 IU/ml resulted in a minimum pH for growth at 5.20, and a minimum aw at 0.967 at the temperature range of 25-35 °C. It was remarkable that low to medium salt concentrations (2.5-4.5 NaCl% w/v) provided a protective effect against inhibition of L. monocytogenes by nisin. The present study points out the applicability of growth/no growth modeling in order to study any interactions between various factors affecting initiation of growth of micro-organisms, in which its turn helps the understudying of microbe-food ecosystem relations and the development of safer food. © 2006 Elsevier Ltd. All rights reserved. en
heal.journalName Food Microbiology en
dc.identifier.issue 8 en
dc.identifier.volume 23 en
dc.identifier.doi 10.1016/j.fm.2006.03.003 en
dc.identifier.spage 779 en
dc.identifier.epage 784 en


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