Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification

dc.contributor.author	Theodoropoulos, G	en
dc.contributor.author	Gazouli, M	en
dc.contributor.author	Ikonomopoulos, JA	en
dc.contributor.author	Kantzoura, V	en
dc.contributor.author	Kominakis, A	en
dc.date.accessioned	2014-06-06T06:47:03Z
dc.date.available	2014-06-06T06:47:03Z
dc.date.issued	2006	en
dc.identifier.issn	03044017	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.vetpar.2005.07.021	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3360
dc.subject	Goats	en
dc.subject	PCR	en
dc.subject	Piroplasms	en
dc.subject	Sheep	en
dc.subject.other	DNA fragment	en
dc.subject.other	ribosome RNA	en
dc.subject.other	article	en
dc.subject.other	Babesia	en
dc.subject.other	babesiosis	en
dc.subject.other	bovids	en
dc.subject.other	controlled study	en
dc.subject.other	dog	en
dc.subject.other	gender	en
dc.subject.other	goat	en
dc.subject.other	Greece	en
dc.subject.other	hemoglobinuria	en
dc.subject.other	herd	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	prevalence	en
dc.subject.other	protozoal infection	en
dc.subject.other	questionnaire	en
dc.subject.other	risk assessment	en
dc.subject.other	risk factor	en
dc.subject.other	sequence homology	en
dc.subject.other	sheep	en
dc.subject.other	tick	en
dc.subject.other	Animals	en
dc.subject.other	Arachnid Vectors	en
dc.subject.other	Babesia	en
dc.subject.other	Babesiosis	en
dc.subject.other	Cattle	en
dc.subject.other	DNA, Protozoan	en
dc.subject.other	Goat Diseases	en
dc.subject.other	Goats	en
dc.subject.other	Greece	en
dc.subject.other	Polymerase Chain Reaction	en
dc.subject.other	Prevalence	en
dc.subject.other	Risk Factors	en
dc.subject.other	RNA, Protozoan	en
dc.subject.other	RNA, Ribosomal, 18S	en
dc.subject.other	Sheep	en
dc.subject.other	Sheep Diseases	en
dc.subject.other	Ticks	en
dc.subject.other	Acari	en
dc.subject.other	Animalia	en
dc.subject.other	Babesia	en
dc.subject.other	Babesia ovis	en
dc.subject.other	Bovidae	en
dc.subject.other	Canis familiaris	en
dc.subject.other	Capra hircus	en
dc.subject.other	Melophagus ovinus	en
dc.subject.other	Ovis aries	en
dc.title	Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.vetpar.2005.07.021	en
heal.publicationDate	2006	en
heal.abstract	A total of 124 blood samples were collected from 92 sheep and 32 goats from 21 randomly selected herds located in two regions of Greece. Data on the characteristics of the animals (species, gender, age, tick burden, presence of haemoglobinuria, prior treatment for babesiosis) and the herd (location, size, species of animals, dogs associated with the herds, tick burden of dogs associated with the herds) were collected through questionnaires. Nineteen animals (15%) produced the DNA fragment specific for Babesia of which 16 were sheep and three were goats. Nucleotide sequence of PCR products revealed 100% homology with Babesia ovis 18S rRNA gene. Nine farms (43%) were found positive for B. ovis. The percentage of positive animals in each farm varied between 10 and 61%. The relative risk of the presence of ticks in sheep and goats (p < 0.01) and farm dogs (p < 0.01) for PCR-positive results for B. ovis in sheep and goats was found 6.63 and 4.14, respectively. © 2005 Elsevier B.V. All rights reserved.	en
heal.journalName	Veterinary Parasitology	en
dc.identifier.issue	2	en
dc.identifier.volume	135	en
dc.identifier.doi	10.1016/j.vetpar.2005.07.021	en
dc.identifier.spage	99	en
dc.identifier.epage	104	en