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Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS

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dc.contributor.author Proestos, C en
dc.contributor.author Sereli, D en
dc.contributor.author Komaitis, M en
dc.date.accessioned 2014-06-06T06:47:03Z
dc.date.available 2014-06-06T06:47:03Z
dc.date.issued 2006 en
dc.identifier.issn 03088146 en
dc.identifier.uri http://dx.doi.org/10.1016/j.foodchem.2004.12.016 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3359
dc.subject Aromatic plants en
dc.subject GC-MS en
dc.subject HPLC en
dc.subject Phenolics en
dc.subject Silylation en
dc.subject.other acetamide derivative en
dc.subject.other aromatic compound en
dc.subject.other caffeic acid en
dc.subject.other catechin en
dc.subject.other chlorotrimethylsilane en
dc.subject.other ferulic acid en
dc.subject.other lavandula vere extract en
dc.subject.other lippia triphylla extract en
dc.subject.other o bis(trimethylsilyl)trifluoroacetamide en
dc.subject.other origanum dictamnus extract en
dc.subject.other phenol derivative en
dc.subject.other plant extract en
dc.subject.other Teucrium polium extract en
dc.subject.other unclassified drug en
dc.subject.other Vitex agnus castus extract en
dc.subject.other article en
dc.subject.other derivatization en
dc.subject.other gas chromatography en
dc.subject.other germander en
dc.subject.other lavender en
dc.subject.other Lippia en
dc.subject.other mass spectrometry en
dc.subject.other nonhuman en
dc.subject.other oregano en
dc.subject.other phytochemistry en
dc.subject.other reversed phase high performance liquid chromatography en
dc.subject.other silylation en
dc.subject.other Vitex agnus castus en
dc.subject.other Aloysia triphylla en
dc.subject.other Lamiaceae en
dc.subject.other Lavandula en
dc.subject.other Lavandula angustifolia en
dc.subject.other Lippia en
dc.subject.other Origanum dictamnus en
dc.subject.other Origanum vulgare en
dc.subject.other Teucrium en
dc.subject.other Teucrium polium en
dc.subject.other Verbenaceae en
dc.subject.other Vitex agnus-castus en
dc.title Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.foodchem.2004.12.016 en
heal.publicationDate 2006 en
heal.abstract It is well known that phenolic compounds are constituents of many plants and herbs, and they have attracted a great deal of public and scientific interest because of their health-promoting effects as antioxidants. Five plants, Vitex agnus-castus (Verbenaceae), Origanum dictamnus (Lamiaceae), Teucrium polium (Lamiaceae), Lavandula vera (Lamiaceae) and Lippia triphylla (Verbenaceae), were examined in order to determine their phenolic composition. Reversed phase high performance liquid chromatography was employed for the identification and quantification of phenolic compounds. Gas chromatography-mass spectometry(GC-MS) was also used for identification of phenolic compounds after silylation. Analysis of the non-volatile and thermolabile phenolic compounds by GC-MS presupposes their conversion into volatile and thermotolerant derivatives. The derivatization process was optimized against reagents, temperature and reaction time. A large excess of N,O-bis(trimethylsilyl) trifluoroacetamide containing trimethylchlorosilane proved to be the best derivatization reagent to convert analytes into volatile trimethylsilyl derivatives. The most abundant phenolic compounds detected were caffeic acid (0.12-0.93 mg 100 g-1 dry sample), ferulic acid (0.34-1.52 mg 100 g-1 dry sample), and (+)-catechin (0.22-0.43 mg 100 g-1 dry sample). © 2005 Elsevier Ltd. All rights reserved. en
heal.journalName Food Chemistry en
dc.identifier.issue 1 en
dc.identifier.volume 95 en
dc.identifier.doi 10.1016/j.foodchem.2004.12.016 en
dc.identifier.spage 44 en
dc.identifier.epage 52 en


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