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Cometabolism of citrate and glucose by Enterococcus faecium FAIR-E 198 in the absence of cellular growth

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dc.contributor.author Vaningelgem, F en
dc.contributor.author Ghijsels, V en
dc.contributor.author Tsakalidou, E en
dc.contributor.author De Vuyst, L en
dc.date.accessioned 2014-06-06T06:47:02Z
dc.date.available 2014-06-06T06:47:02Z
dc.date.issued 2006 en
dc.identifier.issn 00992240 en
dc.identifier.uri http://dx.doi.org/10.1128/AEM.72.1.319-326.2006 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3349
dc.subject.other Cells en
dc.subject.other Ethanol en
dc.subject.other Glucose en
dc.subject.other Growth kinetics en
dc.subject.other Metabolism en
dc.subject.other Acetoin en
dc.subject.other Citrate metabolism en
dc.subject.other Citrates en
dc.subject.other Enterococcus faecium en
dc.subject.other Bacteria en
dc.subject.other 2,3 butanedione en
dc.subject.other acetic acid en
dc.subject.other acetoin en
dc.subject.other alcohol en
dc.subject.other carbon dioxide en
dc.subject.other citric acid en
dc.subject.other formic acid en
dc.subject.other glucose en
dc.subject.other ethanol en
dc.subject.other glucose en
dc.subject.other metabolism en
dc.subject.other article en
dc.subject.other bacterial metabolism en
dc.subject.other bacterial strain en
dc.subject.other cell free system en
dc.subject.other cell growth en
dc.subject.other cheese en
dc.subject.other concentration (parameters) en
dc.subject.other controlled study en
dc.subject.other culture medium en
dc.subject.other energy metabolism en
dc.subject.other energy yield en
dc.subject.other Enterococcus faecium en
dc.subject.other fermentation en
dc.subject.other glucose metabolism en
dc.subject.other nonhuman en
dc.subject.other pH en
dc.subject.other cheese en
dc.subject.other growth, development and aging en
dc.subject.other metabolism en
dc.subject.other microbiology en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Enterococcus faecium en
dc.subject.other Cheese en
dc.subject.other Citrates en
dc.subject.other Culture Media en
dc.subject.other Enterococcus faecium en
dc.subject.other Fermentation en
dc.subject.other Glucose en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Industrial Microbiology en
dc.title Cometabolism of citrate and glucose by Enterococcus faecium FAIR-E 198 in the absence of cellular growth en
heal.type journalArticle en
heal.identifier.primary 10.1128/AEM.72.1.319-326.2006 en
heal.publicationDate 2006 en
heal.abstract Citrate metabolism by Enterococcus faecium FMR-E 198, an isolate from Greek Feta cheese, was studied in modified MRS (mMRS) medium under different pH conditions and glucose and citrate concentrations. In the absence of glucose, this strain was able to metabolize citrate in a pH range from constant pH 5.0 to 7.0. At a constant pH 8.0, no citrate was metabolized, although growth took place. The main end products of citrate metabolism were acetate, formate, acetoin, and carbon dioxide, whereas ethanol and diacetyl were present in smaller amounts. In the presence of glucose, citrate was cometabolized, but it did not contribute to growth. Also, more acetate and less acetoin were formed compared to growth in mMRS medium and in the absence of glucose. Most of the citrate was consumed during the stationary phase, indicating that energy generated by citrate metabolism was used for maintenance. Experiments with cell-free fermented mMRS medium indicated that E. faecium FAIR-E 198 was able to metabolize another energy source present in the medium. Copyright © 2006, American Society for Microbiology. All Rights Reserved. en
heal.journalName Applied and Environmental Microbiology en
dc.identifier.issue 1 en
dc.identifier.volume 72 en
dc.identifier.doi 10.1128/AEM.72.1.319-326.2006 en
dc.identifier.spage 319 en
dc.identifier.epage 326 en


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