| dc.contributor.author |
Moschopoulou, G |
en |
| dc.contributor.author |
Kintzios, S |
en |
| dc.date.accessioned |
2014-06-06T06:47:00Z |
|
| dc.date.available |
2014-06-06T06:47:00Z |
|
| dc.date.issued |
2006 |
en |
| dc.identifier.issn |
00032670 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.aca.2006.01.089 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3335 |
|
| dc.subject |
Bioelectric recognition assay |
en |
| dc.subject |
Cell biosensor |
en |
| dc.subject |
Hydrogen peroxide |
en |
| dc.subject |
Membrane-engineering |
en |
| dc.subject |
Superoxide |
en |
| dc.subject |
Superoxide dismutase |
en |
| dc.subject.other |
Bioassay |
en |
| dc.subject.other |
Biosensors |
en |
| dc.subject.other |
Cells |
en |
| dc.subject.other |
Electrophysiology |
en |
| dc.subject.other |
Enzyme kinetics |
en |
| dc.subject.other |
Hydrogen peroxide |
en |
| dc.subject.other |
Negative ions |
en |
| dc.subject.other |
Bioelectric recognition assay |
en |
| dc.subject.other |
Cell biosensor |
en |
| dc.subject.other |
Membrane-engineering |
en |
| dc.subject.other |
Superoxide dismutase (SOD) |
en |
| dc.subject.other |
Superoxides |
en |
| dc.subject.other |
Biological membranes |
en |
| dc.subject.other |
alginic acid |
en |
| dc.subject.other |
oxygen |
en |
| dc.subject.other |
superoxide |
en |
| dc.subject.other |
superoxide dismutase |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bioenergy |
en |
| dc.subject.other |
bioengineering |
en |
| dc.subject.other |
biosensor |
en |
| dc.subject.other |
catalysis |
en |
| dc.subject.other |
cell membrane potential |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
electrochemistry |
en |
| dc.subject.other |
mammal cell |
en |
| dc.subject.other |
membrane |
en |
| dc.subject.other |
microelectrode |
en |
| dc.subject.other |
molecular recognition |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
room temperature |
en |
| dc.subject.other |
storage |
en |
| dc.subject.other |
Vero cell |
en |
| dc.subject.other |
Mammalia |
en |
| dc.title |
Application of ""membrane-engineering"" to bioelectric recognition cell sensors for the ultra-sensitive detection of superoxide radical: A novel biosensor principle |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.aca.2006.01.089 |
en |
| heal.publicationDate |
2006 |
en |
| heal.abstract |
A new, hybrid type of ultra-sensitive electrophysiological superoxide anion (O2{radical dot}-) sensor is described, which is based on ""membrane-engineered"" mammalian cells immobilized in an alginate matrix. The membrane-engineering process involved the electroinsertion of superoxide dismutase (SOD) molecules in the membranes of Vero fibroblast cells, which acted as catalytic units able to convert O2{radical dot}- to H2O2. Superoxide dismutation triggered changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the bioelectric recognition assay (BERA). The sensor instantly responded to O2{radical dot}- with a detection limit (S/N = 3) of 100 pM. Combined with a 4-month storage capacity at room temperature, the novel biosensor principle offers new perspectives for monitoring ultra-low concentrations of free radical species and oxidative agents in biological systems. © 2006 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Analytica Chimica Acta |
en |
| dc.identifier.volume |
573-574 |
en |
| dc.identifier.doi |
10.1016/j.aca.2006.01.089 |
en |
| dc.identifier.spage |
90 |
en |
| dc.identifier.epage |
96 |
en |