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A severe Hellenic CMV tomato isolate: Symptom variability in tobacco, characterization and discrimination of variants

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dc.contributor.author Sclavounos, AP en
dc.contributor.author Voloudakis, AE en
dc.contributor.author Arabatzis, Ch en
dc.contributor.author Kyriakopoulou, PE en
dc.date.accessioned 2014-06-06T06:46:57Z
dc.date.available 2014-06-06T06:46:57Z
dc.date.issued 2006 en
dc.identifier.issn 09291873 en
dc.identifier.uri http://dx.doi.org/10.1007/s10658-006-0010-8 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3327
dc.subject Aphid transmission en
dc.subject CMV en
dc.subject CMV-variants en
dc.subject Cross protection en
dc.subject Host range en
dc.subject Symptom variability en
dc.subject.other aphid en
dc.subject.other fruit en
dc.subject.other host-parasite interaction en
dc.subject.other phenotype en
dc.subject.other tobacco en
dc.subject.other viral disease en
dc.subject.other Eurasia en
dc.subject.other Europe en
dc.subject.other Greece en
dc.subject.other Southern Europe en
dc.subject.other Aphididae en
dc.subject.other Chenopodium quinoa en
dc.subject.other Cucumber mosaic virus en
dc.subject.other Cucurbita pepo var. melopepo en
dc.subject.other Lycopersicon esculentum en
dc.subject.other Magnoliophyta en
dc.subject.other Nicotiana obtusifolia en
dc.subject.other Nicotiana tabacum en
dc.subject.other Solanaceae en
dc.title A severe Hellenic CMV tomato isolate: Symptom variability in tobacco, characterization and discrimination of variants en
heal.type journalArticle en
heal.identifier.primary 10.1007/s10658-006-0010-8 en
heal.publicationDate 2006 en
heal.abstract A severe strain of Cucumber mosaic virus (CMV) originating from an infected tomato plant (Gastouni-Olympia, Greece) was isolated in tobacco (Nicotiana tabacum cv. Xanthi nc), after three serial local lesion passages in Chenopodium quinoa and designated CMV-G. CMV-G induces yellow mosaic (YM) symptoms in tobacco. When CMV-G was passed mechanically through C. quinoa, phenotypic variants inducing YM or green mild mosaic (MM) in tobacco were isolated. Aphid transmission, from different hosts, appears to be an effective approach for separating MM variants of CMV-G from YM variants. In particular, aphid transmission from zucchini proved to be very efficient in selecting for MM variants. In contrast, aphids transmitted only YM variants from tomato plants. Molecular characterization of CMV-G and its progeny resulted in their classification in the CMV subgroup IB, free of satellite RNA, being the first discovery of the subgroup IB in Greece. In the Solanaceae family (tobacco, tomato, pepper) YM variants induced more severe symptoms than the MM variants. YM and MM phenotype was stable in tobacco for all seven passages tried using the obtained YM and MM variants. Cross-protection experiments showed that an isolated MM variant was able to protect tobacco plants against a challenge infection by a YM variant. © Springer 2006. en
heal.journalName European Journal of Plant Pathology en
dc.identifier.issue 2 en
dc.identifier.volume 115 en
dc.identifier.doi 10.1007/s10658-006-0010-8 en
dc.identifier.spage 163 en
dc.identifier.epage 172 en


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