| dc.contributor.author |
Chorianopoulos, NG |
en |
| dc.contributor.author |
Lambert, RJW |
en |
| dc.contributor.author |
Skandamis, PN |
en |
| dc.contributor.author |
Evergetis, ET |
en |
| dc.contributor.author |
Haroutounian, SA |
en |
| dc.contributor.author |
Nychas, G-JE |
en |
| dc.date.accessioned |
2014-06-06T06:46:57Z |
|
| dc.date.available |
2014-06-06T06:46:57Z |
|
| dc.date.issued |
2006 |
en |
| dc.identifier.issn |
13645072 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1365-2672.2006.02827.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3324 |
|
| dc.subject |
Absorbance |
en |
| dc.subject |
Conductance |
en |
| dc.subject |
Impedance |
en |
| dc.subject |
Inhibition |
en |
| dc.subject |
MIC |
en |
| dc.subject |
Natural antimicrobials |
en |
| dc.subject |
Satureja |
en |
| dc.subject.other |
essential oil |
en |
| dc.subject.other |
antimicrobial activity |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Bacillus cereus |
en |
| dc.subject.other |
bacterium colony |
en |
| dc.subject.other |
colony forming unit |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
electric conductivity |
en |
| dc.subject.other |
Escherichia coli O157 |
en |
| dc.subject.other |
impedance |
en |
| dc.subject.other |
inoculation |
en |
| dc.subject.other |
linear regression analysis |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.subject.other |
microorganism detection |
en |
| dc.subject.other |
minimum inhibitory concentration |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
optical density |
en |
| dc.subject.other |
Salmonella enterica |
en |
| dc.subject.other |
Salmonella enteritidis |
en |
| dc.subject.other |
savory |
en |
| dc.subject.other |
screening test |
en |
| dc.subject.other |
serotype |
en |
| dc.subject.other |
Staphylococcus aureus |
en |
| dc.subject.other |
statistical analysis |
en |
| dc.subject.other |
Student t test |
en |
| dc.subject.other |
Anti-Bacterial Agents |
en |
| dc.subject.other |
Bacillus cereus |
en |
| dc.subject.other |
Colony Count, Microbial |
en |
| dc.subject.other |
Escherichia coli O157 |
en |
| dc.subject.other |
Food Microbiology |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.subject.other |
Microbial Sensitivity Tests |
en |
| dc.subject.other |
Oils, Volatile |
en |
| dc.subject.other |
Salmonella enterica |
en |
| dc.subject.other |
Salmonella enteritidis |
en |
| dc.subject.other |
Satureja |
en |
| dc.subject.other |
Staphylococcus aureus |
en |
| dc.subject.other |
Bacillus cereus |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Enteritidis |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Escherichia coli O157:H7 |
en |
| dc.subject.other |
Listeria monocytogenes |
en |
| dc.subject.other |
Salmonella |
en |
| dc.subject.other |
Salmonella enterica |
en |
| dc.subject.other |
Salmonella enteritidis |
en |
| dc.subject.other |
Satureja |
en |
| dc.subject.other |
Staphylococcus aureus |
en |
| dc.title |
A newly developed assay to study the minimum inhibitory concentration of Satureja spinosa essential oil |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1365-2672.2006.02827.x |
en |
| heal.publicationDate |
2006 |
en |
| heal.abstract |
Aims: The minimum inhibitory concentration (MIC) of Satureja spinosa essential oil against Staphylococcus aureus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, Salmonella serovar Enteritidis PT4 and Bacillus cereus was comparatively assessed with an established optical density method as well as a novel impedimetric method. Methods and Results: The impedimetric analysis takes into account information of microbial growth, such as detection time, maximum conductance, and slope of the conductance curve. For each pathogen two levels of inoculation were studied, a high (10 5 CFU ml -1) and a low level (10 2 CFU ml -1). Non-linear regression analysis was used to fit the data using a modification of a previously published model, from which a more exact value can be obtained for the MIC. Both methods gave similar MICs as shown by t-test statistical analysis. Salm. Enteritidis seems to be the least sensitive to the action of S. spinosa essential oil followed by L. monocytogenes, E. coli, B.cereus and Staph. aureus. The MICs of low inoculum were lower than that of high inoculum. Conclusions: The new impedimetric assay of MIC of essential oils can be considered a reliable rapid method for screening antimicrobial effectiveness of natural additives. Significance and Impact of the Study: Determination of the minimum inhibitory concentration of an essential oil with the simple conductance technique and further study of the mode of action of its components is a good combination for obtaining additional knowledge for industrial application of such natural additives. © 2006 The Society for Applied Microbiology. |
en |
| heal.journalName |
Journal of Applied Microbiology |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
100 |
en |
| dc.identifier.doi |
10.1111/j.1365-2672.2006.02827.x |
en |
| dc.identifier.spage |
778 |
en |
| dc.identifier.epage |
786 |
en |