Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP

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dc.contributor.author Arabatzis, M en
dc.contributor.author Xylouri, E en
dc.contributor.author Frangiadaki, I en
dc.contributor.author Tzimogianni, A en
dc.contributor.author Milioni, A en
dc.contributor.author Arsenis, G en
dc.contributor.author Velegraki, A en
dc.date.accessioned 2014-06-06T06:46:50Z
dc.date.available 2014-06-06T06:46:50Z
dc.date.issued 2006 en
dc.identifier.issn 09594493 en
dc.identifier.uri http://dx.doi.org/10.1111/j.1365-3164.2006.00535.x en
dc.subject.other animal en
dc.subject.other animal disease en
dc.subject.other Arthroderma en
dc.subject.other Arthrodermataceae en
dc.subject.other article en
dc.subject.other dermatomycosis en
dc.subject.other isolation and purification en
dc.subject.other methodology en
dc.subject.other microbiology en
dc.subject.other polymerase chain reaction en
dc.subject.other rabbit en
dc.subject.other restriction fragment length polymorphism en
dc.subject.other skin en
dc.subject.other Animals en
dc.subject.other Arthrodermataceae en
dc.subject.other Dermatomycoses en
dc.subject.other Polymerase Chain Reaction en
dc.subject.other Polymorphism, Restriction Fragment Length en
dc.subject.other Rabbits en
dc.subject.other Skin en
dc.subject.other Arthroderma en
dc.subject.other Arthroderma benhamiae en
dc.subject.other Arthroderma vanbreuseghemii en
dc.subject.other Oryctolagus cuniculus en
dc.title Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP en
heal.type journalArticle en
heal.identifier.primary 10.1111/j.1365-3164.2006.00535.x en
heal.publicationDate 2006 en
heal.abstract Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding Ddel restriction profiles of the ITS1/4 PCR products matched one of the two published T. mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct Ddel restriction sites for Arthroderma benhamiae and A. vanbreuseghemii, while all Ddel-generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification. © 2006 European Society of Veterinary Dermatology. en
heal.journalName Veterinary Dermatology en
dc.identifier.issue 5 en
dc.identifier.volume 17 en
dc.identifier.doi 10.1111/j.1365-3164.2006.00535.x en
dc.identifier.spage 322 en
dc.identifier.epage 326 en

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