| dc.contributor.author |
Arabatzis, M |
en |
| dc.contributor.author |
Xylouri, E |
en |
| dc.contributor.author |
Frangiadaki, I |
en |
| dc.contributor.author |
Tzimogianni, A |
en |
| dc.contributor.author |
Milioni, A |
en |
| dc.contributor.author |
Arsenis, G |
en |
| dc.contributor.author |
Velegraki, A |
en |
| dc.date.accessioned |
2014-06-06T06:46:50Z |
|
| dc.date.available |
2014-06-06T06:46:50Z |
|
| dc.date.issued |
2006 |
en |
| dc.identifier.issn |
09594493 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1365-3164.2006.00535.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3222 |
|
| dc.subject.other |
animal |
en |
| dc.subject.other |
animal disease |
en |
| dc.subject.other |
Arthroderma |
en |
| dc.subject.other |
Arthrodermataceae |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
dermatomycosis |
en |
| dc.subject.other |
isolation and purification |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
microbiology |
en |
| dc.subject.other |
polymerase chain reaction |
en |
| dc.subject.other |
rabbit |
en |
| dc.subject.other |
restriction fragment length polymorphism |
en |
| dc.subject.other |
skin |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Arthrodermataceae |
en |
| dc.subject.other |
Dermatomycoses |
en |
| dc.subject.other |
Polymerase Chain Reaction |
en |
| dc.subject.other |
Polymorphism, Restriction Fragment Length |
en |
| dc.subject.other |
Rabbits |
en |
| dc.subject.other |
Skin |
en |
| dc.subject.other |
Arthroderma |
en |
| dc.subject.other |
Arthroderma benhamiae |
en |
| dc.subject.other |
Arthroderma vanbreuseghemii |
en |
| dc.subject.other |
Oryctolagus cuniculus |
en |
| dc.title |
Rapid detection of Arthroderma vanbreuseghemii in rabbit skin specimens by PCR-RFLP |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1365-3164.2006.00535.x |
en |
| heal.publicationDate |
2006 |
en |
| heal.abstract |
Three commercial rabbit farms independently reported suspected dermatophyte infections during 2004. Cultures confirmed that the infection was caused by the zoophilic Tricophyton mentagrophytes, while mating studies and sequencing of the internal transcribed spacer (ITS)1/4 polymerase chain reaction products, directly amplified from skin lesions and from pure culture isolates, established its Arthroderma vanbreuseghemii lineage. Subsequently, the corresponding Ddel restriction profiles of the ITS1/4 PCR products matched one of the two published T. mentagrophytes complex profiles. Sequence analysis of the published ITS sequences predicted distinct Ddel restriction sites for Arthroderma benhamiae and A. vanbreuseghemii, while all Ddel-generated restriction fragment length polymorphisms of the rabbit isolates were identical to those of A. vanbreuseghemii. The proposed procedure can be used for rapid dermatophyte detection and presumptive T. mentagrophytes identification. © 2006 European Society of Veterinary Dermatology. |
en |
| heal.journalName |
Veterinary Dermatology |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
17 |
en |
| dc.identifier.doi |
10.1111/j.1365-3164.2006.00535.x |
en |
| dc.identifier.spage |
322 |
en |
| dc.identifier.epage |
326 |
en |