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Cytological and other aspects of pathogenesis-related gene expression in tomato plants grown on a suppressive compost

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dc.contributor.author Kavroulakis, N en
dc.contributor.author Papadopoulou, KK en
dc.contributor.author Ntougias, S en
dc.contributor.author Zervakis, GI en
dc.contributor.author Ehaliotis, C en
dc.date.accessioned 2014-06-06T06:46:45Z
dc.date.available 2014-06-06T06:46:45Z
dc.date.issued 2006 en
dc.identifier.issn 03057364 en
dc.identifier.uri http://dx.doi.org/10.1093/aob/mcl149 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3186
dc.subject Compost en
dc.subject Induced resistance en
dc.subject P69 en
dc.subject Pathogenesis-related (PR) proteins en
dc.subject PR-1 en
dc.subject PR-5 en
dc.subject Solanum lycopersicum en
dc.subject Tomato en
dc.subject.other pathogenesis related proteins, plant en
dc.subject.other pathogenesis-related proteins, plant en
dc.subject.other vegetable protein en
dc.subject.other compost en
dc.subject.other cytology en
dc.subject.other disease resistance en
dc.subject.other fruit en
dc.subject.other gene expression en
dc.subject.other induced response en
dc.subject.other pathogenicity en
dc.subject.other plant defense en
dc.subject.other root en
dc.subject.other article en
dc.subject.other cytology en
dc.subject.other gene expression regulation en
dc.subject.other genetics en
dc.subject.other growth, development and aging en
dc.subject.other in situ hybridization en
dc.subject.other molecular genetics en
dc.subject.other nucleotide sequence en
dc.subject.other plant root en
dc.subject.other plant stem en
dc.subject.other reverse transcription polymerase chain reaction en
dc.subject.other sequence alignment en
dc.subject.other soil en
dc.subject.other tomato en
dc.subject.other Base Sequence en
dc.subject.other Gene Expression Regulation, Plant en
dc.subject.other In Situ Hybridization en
dc.subject.other Lycopersicon esculentum en
dc.subject.other Molecular Sequence Data en
dc.subject.other Plant Proteins en
dc.subject.other Plant Roots en
dc.subject.other Plant Stems en
dc.subject.other Reverse Transcriptase Polymerase Chain Reaction en
dc.subject.other Sequence Alignment en
dc.subject.other Soil en
dc.subject.other Lycopersicon esculentum en
dc.subject.other Solanum en
dc.title Cytological and other aspects of pathogenesis-related gene expression in tomato plants grown on a suppressive compost en
heal.type journalArticle en
heal.identifier.primary 10.1093/aob/mcl149 en
heal.publicationDate 2006 en
heal.abstract • Background and Aims: Recent studies have shown that certain composts may trigger indirect defence mechanisms by sensitizing the plant to create an increased state of resistance, similar to systemic acquired resistance. In this study, the capacity of a disease-suppressive compost to alter the expression pattern of certain pathogenesis-related (PR) genes in the root system of tomato plants (Solanum lycopersicum) provided the opportunity to study their cellular expression pattern and to investigate putative roles of these genes in the mechanisms of plant defence. • Methods: Employing the reverse transcription-polymerase chain reaction (RT-PCR) and in situ RNA:RNA hybridization techniques, the accumulation and distribution of the transcripts of the differentially expressed PR genes were examined in plants grown on compost and compared with those of control plants grown on peat. • Key Results: Elevated levels of expression of the pathogenesis-related genes PR-1, PR-5 and P69/PR-7 were detected in the roots of tomato plants grown on the compost. A clearly distinguished spatial induction pattern was observed for these PR genes: PR-1 transcripts were almost exclusively detected in the pericycle cells surrounding the root stele of the main and lateral roots; PR-5 transcripts were present in the phloem of the root and stem tissues; and the accumulation and distribution of PR-7 transcripts was detected in discrete groups of cells that appeared sporadically in both the parenchyma and vascular system of the root, suggesting that the gene is not expressed in a tissue-specific manner. In addition, a novel cDNA clone was isolated (P69G), which probably encodes a new tomato P69 isoform. • Conclusions: This study provides evidence that a supressive compost is able to elicit consistent and increased expression of certain PR genes in the roots of tomato plants, even in the absence of any pathogen. The in situ localization studies reveal expression patterns which are in accordance with the presence of protein or with the putative roles of the respective encoded proteins. The expression of the PR genes may be triggered by the microflora of the compost or could be associated with abiotic factors of the compost. © The Author 2006. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. en
heal.journalName Annals of Botany en
dc.identifier.issue 3 en
dc.identifier.volume 98 en
dc.identifier.doi 10.1093/aob/mcl149 en
dc.identifier.spage 555 en
dc.identifier.epage 564 en


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