Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.)

dc.contributor.author	Banilas, G	en
dc.contributor.author	Moressis, A	en
dc.contributor.author	Nikoloudakis, N	en
dc.contributor.author	Hatzopoulos, P	en
dc.date.accessioned	2014-06-06T06:46:44Z
dc.date.available	2014-06-06T06:46:44Z
dc.date.issued	2005	en
dc.identifier.issn	0168-9452	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3168
dc.subject	FAD2	en
dc.subject	FAD6	en
dc.subject	fatty acid desaturase	en
dc.subject	olive	en
dc.subject	spatial expression	en
dc.subject	temporal expression	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Plant Sciences	en
dc.subject.other	FATTY-ACID DESATURASE	en
dc.subject.other	CARRIER PROTEIN DESATURASE	en
dc.subject.other	ARABIDOPSIS FAD2 GENE	en
dc.subject.other	FUNCTIONAL EXPRESSION	en
dc.subject.other	MOLECULAR ANALYSIS	en
dc.subject.other	CLONING	en
dc.subject.other	MUTANT	en
dc.subject.other	CDNA	en
dc.subject.other	PURIFICATION	en
dc.subject.other	TEMPERATURE	en
dc.title	Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.)	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	Two cDNAs, OeFAD2 and OeFAD6, encoding omega-6 fatty acid desaturases, the key enzymes for the conversion of oleic into linoleic acid, were isolated from olive. OeFAD2 contains a C-terminal ER retrieval motif, whereas OeFAD6 possess a putative N-terminal plastidial signal peptide. The deduced amino acid sequence of OeFAD2 showed higher similarities to other plant microsomal omega-6 desasurases than to OeFAD6 or plastidial orthologues, and vice versa. Southern analysis indicated that the OeFAD2 gene is represented by one or two copies and OeFAD6 by a single copy gene. Expression analysis by RT-PCR showed that both genes are expressed in all tissues of olive tree tested, but higher levels of mRNA accumulation were detected in reproductive organs and cells that proliferate rapidly or store lipids. The two genes exhibited distinct patterns of mRNA accumulation during olive drupe growth. OeFAD2 was constitutively expressed, with maximum transcript accumulation in mesocarps, whereas OeFAD6 was developmentally regulated in both endosperms and embryos. The expression patterns observed reflect the discrete roles of these genes in membrane synthesis for cell division, thylakoid development, and lipid storage or in the biosynthetic pathway for the production of signaling molecules that influence plant development or defense. (C) 2004 Elsevier Ireland Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI IRELAND LTD	en
heal.journalName	PLANT SCIENCE	en
dc.identifier.issue	2	en
dc.identifier.volume	168	en
dc.identifier.isi	ISI:000226558800033	en
dc.identifier.spage	547	en
dc.identifier.epage	555	en