The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: Association with α-tocopherol, H2O2 and size of culture vessel

dc.contributor.author	Matakiadis, T	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:46:40Z
dc.date.available	2014-06-06T06:46:40Z
dc.date.issued	2005	en
dc.identifier.issn	01676903	en
dc.identifier.uri	http://dx.doi.org/10.1007/s10725-005-1046-z	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3129
dc.subject	α-tocopherol	en
dc.subject	Adenosine 5′-triphosphate	en
dc.subject	Bioreactor	en
dc.subject	Cucumis sativus	en
dc.subject	Liquid culture	en
dc.subject.other	Antioxidants	en
dc.subject.other	Bioreactors	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Cell culture	en
dc.subject.other	Cost effectiveness	en
dc.subject.other	Enzymes	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Cucumber	en
dc.subject.other	Liquid culture vessels	en
dc.subject.other	Nodal explants	en
dc.subject.other	Root length	en
dc.subject.other	Adenosinetriphosphate	en
dc.subject.other	Adenosine	en
dc.subject.other	Antioxidants	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Cost Effectiveness	en
dc.subject.other	Enzymes	en
dc.subject.other	Phosphates	en
dc.subject.other	Plants	en
dc.subject.other	Cucumis sativus	en
dc.title	The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: Association with α-tocopherol, H2O2 and size of culture vessel	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10725-005-1046-z	en
heal.publicationDate	2005	en
heal.abstract	We investigated the effect of adenosine 5′-triphosphate (ATP), α-tocopherol and H202 on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. The effect of the size of the liquid culture vessel (250-ml flask vs. 2.5-l airlift bioreactor) was also evaluated. The addition of ATP alone caused a significant increase in the number of branches (internodes) and in internodal length, but also a reduction of leaf number, compared to control cultures. It also increased significantly the accumulation of NO3 - and K+ . The application of ATP + α-tocopherol was associated with a significant increase in bud, internodal, leaf and petiole number, internodal, petiole and root length, as well as plant fresh weight, but reduced PO4 3- and Ca 2+ accumulation. The combined application of ATP + α-tocopherol + H2O2 was associated with maximum petiole length and increased plant fresh weight but reduced Ca2+ accumulation. Compared to all other treatments, application of ATP + α-tocopherol in bioreactor-incubated cultures produced significantly larger plants, with an increased bud number, internode lenght and soluble carbohydrate concentration, but also with a reduced fresh weight, root length and reduced NO3 - and PO4 3- and Ca2+ concentrations. These effects were associated with changes in the redox status of the regenerants, as well as dehydrogenase and peroxidase activities. The perspective for an application of ATP and antioxidants as novel, cost-efficient growth regulators in the micropropagation of this commercially important vegetable species is discussed. © Springer 2005.	en
heal.journalName	Plant Growth Regulation	en
dc.identifier.issue	2	en
dc.identifier.volume	45	en
dc.identifier.doi	10.1007/s10725-005-1046-z	en
dc.identifier.spage	127	en
dc.identifier.epage	137	en