RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity

dc.contributor.author	Proestos, C	en
dc.contributor.author	Chorianopoulos, N	en
dc.contributor.author	Nychas, G-JE	en
dc.contributor.author	Komaitis, M	en
dc.date.accessioned	2014-06-06T06:46:38Z
dc.date.available	2014-06-06T06:46:38Z
dc.date.issued	2005	en
dc.identifier.issn	00218561	en
dc.identifier.uri	http://dx.doi.org/10.1021/jf040083t	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3116
dc.subject	Antimicrobial activity	en
dc.subject	Aromatic plants	en
dc.subject	Phenolic antioxidants	en
dc.subject	RP-HPLC	en
dc.subject	Total phenolics	en
dc.subject.other	4 hydroxybenzoic acid	en
dc.subject.other	apigenin	en
dc.subject.other	caffeic acid	en
dc.subject.other	catechin	en
dc.subject.other	coumaric acid	en
dc.subject.other	epicatechin	en
dc.subject.other	eriodictyol	en
dc.subject.other	ferulic acid	en
dc.subject.other	flavonoid	en
dc.subject.other	gallic acid	en
dc.subject.other	gentisic acid	en
dc.subject.other	luteolin	en
dc.subject.other	naringenin	en
dc.subject.other	phenol derivative	en
dc.subject.other	plant extract	en
dc.subject.other	quercetin	en
dc.subject.other	sunflower oil	en
dc.subject.other	syringic acid	en
dc.subject.other	antimicrobial activity	en
dc.subject.other	antioxidant activity	en
dc.subject.other	aromatic plant	en
dc.subject.other	article	en
dc.subject.other	Bacillus cereus	en
dc.subject.other	chemical analysis	en
dc.subject.other	Escherichia coli	en
dc.subject.other	extraction	en
dc.subject.other	hydrolysis	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Menta pulegium	en
dc.subject.other	minimum inhibitory concentration	en
dc.subject.other	nonhuman	en
dc.subject.other	plant	en
dc.subject.other	reversed phase high performance liquid chromatography	en
dc.subject.other	Salmonella enteritidis	en
dc.subject.other	Staphylococcus aureus	en
dc.subject.other	thyme	en
dc.subject.other	Anti-Bacterial Agents	en
dc.subject.other	Antioxidants	en
dc.subject.other	Caffeic Acids	en
dc.subject.other	Chromatography, High Pressure Liquid	en
dc.subject.other	Coumaric Acids	en
dc.subject.other	Phenols	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Helianthus	en
dc.subject.other	Menta	en
dc.subject.other	Thymus vulgaris	en
dc.title	RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/jf040083t	en
heal.publicationDate	2005	en
heal.abstract	Extracts of aromatic plants of Greek origin were examined as potential sources of phenolic compounds. RP-HPLC with UV detection was employed for the identification and quantification of the phenolic antioxidants, present in methanolic extracts. The most abundant phenolic acids were ferulic acid (1.1-280 mg/100 g of dry sample) and caffeic acid (1.2-60 mg/100 g of dry sample). (+)-Catechin and quercetin were the most abundant flavonoids. Apigenin and luteolin were detected in high amounts in Menta pulegium and Thymus vulgaris, respectively. The antioxidant capacity was determined, in dried ground plants and in their methanol extracts, with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenolic content in the extracts was determined spectrometrically according to the Folin-Ciocalteu assay and ranged from 1 to 21 mg of gallic acid/100 g of dry sample. Antimicrobial activity of the extracts against selected microbes was also conducted in this study.	en
heal.journalName	Journal of Agricultural and Food Chemistry	en
dc.identifier.issue	4	en
dc.identifier.volume	53	en
dc.identifier.doi	10.1021/jf040083t	en
dc.identifier.spage	1190	en
dc.identifier.epage	1195	en