| dc.contributor.author |
Labrinea, EP |
en |
| dc.contributor.author |
Georgiou, CA |
en |
| dc.date.accessioned |
2014-06-06T06:46:37Z |
|
| dc.date.available |
2014-06-06T06:46:37Z |
|
| dc.date.issued |
2005 |
en |
| dc.identifier.issn |
00218561 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1021/jf050106j |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3113 |
|
| dc.subject |
ABTS•+ |
en |
| dc.subject |
Flow injection |
en |
| dc.subject |
Gradient dilution |
en |
| dc.subject |
Total antioxidant capacity |
en |
| dc.subject.other |
2,2' azino di (3 ethylbenzothiazoline) 6 sulfonic acid |
en |
| dc.subject.other |
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid |
en |
| dc.subject.other |
antioxidant |
en |
| dc.subject.other |
benzothiazole derivative |
en |
| dc.subject.other |
dyes, reagents, indicators, markers and buffers |
en |
| dc.subject.other |
sulfonic acid derivative |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
autoanalysis |
en |
| dc.subject.other |
flow injection analysis |
en |
| dc.subject.other |
honey |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
pH |
en |
| dc.subject.other |
sensitivity and specificity |
en |
| dc.subject.other |
wine |
en |
| dc.subject.other |
Antioxidants |
en |
| dc.subject.other |
Autoanalysis |
en |
| dc.subject.other |
Benzothiazoles |
en |
| dc.subject.other |
Flow Injection Analysis |
en |
| dc.subject.other |
Honey |
en |
| dc.subject.other |
Hydrogen-Ion Concentration |
en |
| dc.subject.other |
Indicators and Reagents |
en |
| dc.subject.other |
Sensitivity and Specificity |
en |
| dc.subject.other |
Sulfonic Acids |
en |
| dc.subject.other |
Wine |
en |
| dc.title |
Rapid, fully automated flow injection antioxidant capacity assay |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1021/jf050106j |
en |
| heal.publicationDate |
2005 |
en |
| heal.abstract |
A flow injection method for antioxidant capacity assessment based on a low-cost laboratory-made analyzer is reported. A sample of 30 μL. is injected in acetate buffer stream, pH 4.6, that converges with ABTS• + reagent stream. Detection is achieved by monitoring absorbance at 414 nm. The proposed method achieves a sample throughput of up to 120 samples h-1, the detection limit being 1.3 μM trolox. Precision was better than 5% relative standard deviation (n = 4) and the linear range was 4-100 μM, expanded to 250 μM trolox utilizing concentration gradients formed along the injected sample bolus. Information on reaction kinetics is obtained through a single injection. The method was applied to pure compounds and wine and honey samples. Good correlation was found between antioxidant capacity assessed through the proposed method and phenolic content: r= 0.94 for red wines, r = 0.96 for white and rose wines, and r = 0.89 for honeys. © 2005 American Chemical Society. |
en |
| heal.journalName |
Journal of Agricultural and Food Chemistry |
en |
| dc.identifier.issue |
11 |
en |
| dc.identifier.volume |
53 |
en |
| dc.identifier.doi |
10.1021/jf050106j |
en |
| dc.identifier.spage |
4341 |
en |
| dc.identifier.epage |
4346 |
en |