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Rapid method for simultaneous quantitative determination of four major essential oil components from oregano (Oreganum sp.) and thyme (Thymus sp.) using FT-Raman spectroscopy

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dc.contributor.author Siatis, NG en
dc.contributor.author Kimbaris, AC en
dc.contributor.author Pappas, CS en
dc.contributor.author Tarantilis, PA en
dc.contributor.author Daferera, DJ en
dc.contributor.author Polissiou, MG en
dc.date.accessioned 2014-06-06T06:46:37Z
dc.date.available 2014-06-06T06:46:37Z
dc.date.issued 2005 en
dc.identifier.issn 00218561 en
dc.identifier.uri http://dx.doi.org/10.1021/jf048930f en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3112
dc.subject Deconvolution en
dc.subject Essential oil en
dc.subject FT-Raman en
dc.subject Oregano en
dc.subject Quantitative en
dc.subject Thyme en
dc.subject.other essential oil en
dc.subject.other accuracy en
dc.subject.other article en
dc.subject.other computer program en
dc.subject.other correlation analysis en
dc.subject.other Fourier transform mass spectrometry en
dc.subject.other gas chromatography en
dc.subject.other intermethod comparison en
dc.subject.other measurement en
dc.subject.other nonhuman en
dc.subject.other oregano en
dc.subject.other quantitative analysis en
dc.subject.other Raman spectrometry en
dc.subject.other thyme en
dc.subject.other Oils, Volatile en
dc.subject.other Origanum en
dc.subject.other Reproducibility of Results en
dc.subject.other Sensitivity and Specificity en
dc.subject.other Software en
dc.subject.other Spectrum Analysis, Raman en
dc.subject.other Thymus Plant en
dc.subject.other Origanum vulgare en
dc.subject.other Thymus vulgaris en
dc.title Rapid method for simultaneous quantitative determination of four major essential oil components from oregano (Oreganum sp.) and thyme (Thymus sp.) using FT-Raman spectroscopy en
heal.type journalArticle en
heal.identifier.primary 10.1021/jf048930f en
heal.publicationDate 2005 en
heal.abstract A simple and rapid method for the quantitative determination of four major components found in oregano and thyme essential oils is presented. The method correlates the Raman peak intensity in the spectral region from 1800 to 600 cm -1 and the concentration percentage of each particular constituent in the sample. To achieve accurate quantification results and avoid the risk of overlapping peaks of unknown Raman-active substances in natural essential oils, the peaks must be analyzed. For this purpose, PEAKSOLVE software (Ver. 1.0.5) was used. Unknown samples were measured with the FT-Raman method, and the results were compared to those of the gas chromatographic (GC) analysis. The comparison was made at a confidence level of 99%, and the two methods scored equally in terms of repeatability and accuracy even at the edge of the method specifications. The new method can provide accurate results in very short times once the setup is complete and could be utilized in areas where vast amounts of samples must be analyzed. en
heal.journalName Journal of Agricultural and Food Chemistry en
dc.identifier.issue 2 en
dc.identifier.volume 53 en
dc.identifier.doi 10.1021/jf048930f en
dc.identifier.spage 202 en
dc.identifier.epage 206 en


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