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Morphometric analysis of root shape

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dc.contributor.author Grabov, A en
dc.contributor.author Ashley, MK en
dc.contributor.author Rigas, S en
dc.contributor.author Hatzopoulos, P en
dc.contributor.author Dolan, L en
dc.contributor.author Vicente-Agullo, F en
dc.date.accessioned 2014-06-06T06:46:35Z
dc.date.available 2014-06-06T06:46:35Z
dc.date.issued 2005 en
dc.identifier.issn 0028646X en
dc.identifier.uri http://dx.doi.org/10.1111/j.1469-8137.2004.01258.x en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3090
dc.subject Auxin en
dc.subject Gravitropism en
dc.subject Image analysis en
dc.subject Root development en
dc.subject Root morphology en
dc.subject.other root system en
dc.subject.other Arabidopsis en
dc.subject.other article en
dc.subject.other biological model en
dc.subject.other gene expression en
dc.subject.other genetics en
dc.subject.other gravitropism en
dc.subject.other growth, development and aging en
dc.subject.other histology en
dc.subject.other mutation en
dc.subject.other plant root en
dc.subject.other Arabidopsis en
dc.subject.other Gene Expression en
dc.subject.other Gravitropism en
dc.subject.other Models, Biological en
dc.subject.other Mutation en
dc.subject.other Plant Roots en
dc.subject.other Arabidopsis en
dc.title Morphometric analysis of root shape en
heal.type journalArticle en
heal.identifier.primary 10.1111/j.1469-8137.2004.01258.x en
heal.publicationDate 2005 en
heal.abstract asterisk inside a circle sign Alterations in the root shape in plant mutants indicate defects in hormonal signalling, transport and cytoskeleton function. asterisk inside a circle sign To quantify the root shape, we introduced novel parameters designated vertical growth index (VGI) and horizontal growth index (HGI). VGI was defined as a ratio between the root tip ordinate and the root length. HGI was the ratio between the root tip abscissa and the root length. asterisk inside a circle sign To assess the applicability of VGI and HGI for quantification of root shape, we analysed root development in agravitropic Arabidopsis mutants. Statistical analysis indicated that VGI is a sensitive morphometric parameter enabling detection of weak gravitropic defects. VGI dynamics were qualitatively similar in auxin-transport mutants aux1, pin2 and trh1, but different in the auxin-signalling mutant axr2. Analysis of VGI and HGI of roots grown on tilted plates showed that the trh1 mutation affected downstream cellular responses rather than perception of the gravitropic stimulus. asterisk inside a circle sign All these tests indicate that the VGI and HGI analysis is a versatile and sensitive method for the study of root morphology. © New Phytologist (2004). en
heal.journalName New Phytologist en
dc.identifier.issue 2 en
dc.identifier.volume 165 en
dc.identifier.doi 10.1111/j.1469-8137.2004.01258.x en
dc.identifier.spage 641 en
dc.identifier.epage 651 en


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