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Molecular characterization and biological response to respiration inhibitors of Pyricularia isolates from ctenanthe and rice plants

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dc.contributor.author Paplomatas, EJ en
dc.contributor.author Pappas, AC en
dc.contributor.author Syranidou, E en
dc.date.accessioned 2014-06-06T06:46:35Z
dc.date.available 2014-06-06T06:46:35Z
dc.date.issued 2005 en
dc.identifier.issn 1526498X en
dc.identifier.uri http://dx.doi.org/10.1002/ps.1036 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3088
dc.subject Alternative oxidase en
dc.subject Fluazinam en
dc.subject Fungicide resistance en
dc.subject Phylogenetic analysis en
dc.subject Pyricularia en
dc.subject Strobilurins en
dc.subject.other acrylic acid derivative en
dc.subject.other aminopyridine derivative en
dc.subject.other azoxystrobin en
dc.subject.other fluazinam en
dc.subject.other fungicide en
dc.subject.other isoenzyme en
dc.subject.other kresoxim methyl en
dc.subject.other kresoxim-methyl en
dc.subject.other methacrylic acid derivative en
dc.subject.other phenylacetic acid derivative en
dc.subject.other pyrimidine derivative en
dc.subject.other salicylamide derivative en
dc.subject.other salicylhydroxamic acid en
dc.subject.other fungicide en
dc.subject.other antibiotic resistance en
dc.subject.other article en
dc.subject.other Ascomycetes en
dc.subject.other comparative study en
dc.subject.other enzymology en
dc.subject.other fungus spore en
dc.subject.other genetics en
dc.subject.other growth, development and aging en
dc.subject.other Marantaceae en
dc.subject.other microbiology en
dc.subject.other oxygen consumption en
dc.subject.other phylogeny en
dc.subject.other physiology en
dc.subject.other rice en
dc.subject.other Acrylates en
dc.subject.other Aminopyridines en
dc.subject.other Ascomycota en
dc.subject.other Drug Resistance, Fungal en
dc.subject.other Fungicides, Industrial en
dc.subject.other Isoenzymes en
dc.subject.other Marantaceae en
dc.subject.other Methacrylates en
dc.subject.other Oryza sativa en
dc.subject.other Oxygen Consumption en
dc.subject.other Phenylacetates en
dc.subject.other Phylogeny en
dc.subject.other Pyrimidines en
dc.subject.other Salicylamides en
dc.subject.other Spores, Fungal en
dc.subject.other Ctenanthe en
dc.subject.other Fungi en
dc.subject.other Magnaporthe grisea en
dc.subject.other Pyricularia en
dc.title Molecular characterization and biological response to respiration inhibitors of Pyricularia isolates from ctenanthe and rice plants en
heal.type journalArticle en
heal.identifier.primary 10.1002/ps.1036 en
heal.publicationDate 2005 en
heal.abstract The molecular profile and the biological response of isolates of Pyricularia oryzae Cavara obtained from ctenanthe to two strobilurins (azoxystrobin, kresoxim-methyl) and the phenylpyridinamine fungicide fluazinam were characterized, and compared with isolates from rice plants. Five different isozymes (α-esterase, lactate, malate, isocitrate and sorbitol dehydrogenases) and five random decamer primers for RAPD-PCR were used to generate molecular markers. Using unweighted pair-group with arithmetic average analysis, ctenanthe isolates were found to form a separate group distinct from that of the rice isolates for both sets of markers. Amplified polymorphic sequences of mitochondrial cytochrome b that were digested with Fnu4HI or StyI revealed no differences among Pyricularia isolates at amino acid positions 143 or 129 which confer resistance to strobilurins in several fungi. In absence of the alternative respiration inhibitor salicylhydroxamic acid (SHAM) the three fungicides showed inferior and variable efficacy, with a trend toward the rice isolate being less sensitive. The addition of SHAM enhanced the effectiveness of all fungicides against isolates regardless of their origin. Appressorium formation was the most vulnerable target of action of the respiration inhibitors and azoxystrobin the most effective. This is the first report of a comparison between the molecular profiles and sensitivities to respiration inhibitors for Pyricularia oryzae isolates from a non-gramineous host and from rice. © 2005 Society of Chemical Industry. en
heal.journalName Pest Management Science en
dc.identifier.issue 7 en
dc.identifier.volume 61 en
dc.identifier.doi 10.1002/ps.1036 en
dc.identifier.spage 691 en
dc.identifier.epage 698 en


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