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Identification of Olea europaea L. cultivars using inter-simple sequence repeat markers

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dc.contributor.author Terzopoulos, PJ en
dc.contributor.author Kolano, B en
dc.contributor.author Bebeli, PJ en
dc.contributor.author Kaltsikes, PJ en
dc.contributor.author Metzidakis, I en
dc.date.accessioned 2014-06-06T06:46:33Z
dc.date.available 2014-06-06T06:46:33Z
dc.date.issued 2005 en
dc.identifier.issn 03044238 en
dc.identifier.uri http://dx.doi.org/10.1016/j.scienta.2005.01.011 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3071
dc.subject Differentiation en
dc.subject Discriminating power en
dc.subject Genetic diversity en
dc.subject ISSRs en
dc.subject Olea europaea en
dc.subject Polymorphic information content en
dc.subject.other cultivar en
dc.subject.other Olea europaea en
dc.title Identification of Olea europaea L. cultivars using inter-simple sequence repeat markers en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.scienta.2005.01.011 en
heal.publicationDate 2005 en
heal.abstract Two inter-simple sequence repeat (ISSR) markers (one UBC-818, rich in CA and the other UBC-849, rich in GT) were used for the differentiation of 31 Olea europaea L. cultivars grown in Greece. Amplification products were visualized using the silver staining technique. Genetic similarities were calculated using the Jaccard similarity coefficient. The resulting similarity matrix was subjected to the UPGMA clustering method for dendrogram construction and cultivar differentiation. Both ISSR primers showed high degrees of polymorphism. Primer UBC-818 yielded more bands (51) than UBC-849 (33) and helped in uniquely identifying all 31 cultivars while the UBC-849 primer led to the identification of only 27. The present results along with those of other researchers show that ISSRs can be used for cultivar differentiation in O. europaea L. © 2005 Elsevier B.V. All rights reserved. en
heal.journalName Scientia Horticulturae en
dc.identifier.issue 1 en
dc.identifier.volume 105 en
dc.identifier.doi 10.1016/j.scienta.2005.01.011 en
dc.identifier.spage 45 en
dc.identifier.epage 51 en


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