Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR - RFLP analysis of three mtDNA segments

dc.contributor.author	Bouga, M	en
dc.contributor.author	Harizanis, PC	en
dc.contributor.author	Kilias, G	en
dc.contributor.author	Alahiotis, S	en
dc.date.accessioned	2014-06-06T06:46:32Z
dc.date.available	2014-06-06T06:46:32Z
dc.date.issued	2005	en
dc.identifier.issn	00448435	en
dc.identifier.uri	http://dx.doi.org/10.1051/apido:2005021	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3064
dc.subject	Apis mellifera	en
dc.subject	Genetic variability	en
dc.subject	Greece	en
dc.subject	Honey bee	en
dc.subject	mtDNA	en
dc.subject.other	divergence	en
dc.subject.other	genetic structure	en
dc.subject.other	honeybee	en
dc.subject.other	population genetics	en
dc.subject.other	Asia	en
dc.subject.other	Cyprus	en
dc.subject.other	Eastern Hemisphere	en
dc.subject.other	Eurasia	en
dc.subject.other	Europe	en
dc.subject.other	Greece	en
dc.subject.other	Middle East	en
dc.subject.other	Southern Europe	en
dc.subject.other	World	en
dc.subject.other	Apidae	en
dc.subject.other	Apis mellifera	en
dc.subject.other	Apis mellifera adami	en
dc.subject.other	Cecropia	en
dc.subject.other	Cypria	en
dc.subject.other	Hymenoptera	en
dc.subject.other	Macedonica	en
dc.title	Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR - RFLP analysis of three mtDNA segments	en
heal.type	journalArticle	en
heal.identifier.primary	10.1051/apido:2005021	en
heal.publicationDate	2005	en
heal.abstract	The genetic structure and phylogenetic relationships among six honey bee populations were studied using RFLP analysis on three PCR-amplified mtDNA gene segments (16s rDNA, CO I, and ND 5). The populations were sampled from various areas of Greece and Cyprus and correspond to Apis mellifera adami, A. m. macedonica, A. m. cecropia, and A. m. cypria races, based on origin (Ruttner, 1988). Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica. © INRA/DIB-AGIB/ EDP Sciences, 2005.	en
heal.journalName	Apidologie	en
dc.identifier.issue	3	en
dc.identifier.volume	36	en
dc.identifier.doi	10.1051/apido:2005021	en
dc.identifier.spage	335	en
dc.identifier.epage	344	en