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FT-Raman spectroscopic simultaneous determination of fructose and glucose in honey

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dc.contributor.author Batsoulis, AN en
dc.contributor.author Siatis, NG en
dc.contributor.author Kimbaris, AC en
dc.contributor.author Alissandrakis, EK en
dc.contributor.author Pappas, CS en
dc.contributor.author Tarantilis, PA en
dc.contributor.author Harizanis, PC en
dc.contributor.author Polissiou, MG en
dc.date.accessioned 2014-06-06T06:46:32Z
dc.date.available 2014-06-06T06:46:32Z
dc.date.issued 2005 en
dc.identifier.issn 00218561 en
dc.identifier.uri http://dx.doi.org/10.1021/jf048793m en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3062
dc.subject Fructose en
dc.subject FT-Raman en
dc.subject Glucose en
dc.subject Honey en
dc.subject Quantitative determination en
dc.subject.other fructose en
dc.subject.other glucose en
dc.subject.other article en
dc.subject.other computer program en
dc.subject.other Fourier transform mass spectrometry en
dc.subject.other glucose assay en
dc.subject.other high performance liquid chromatography en
dc.subject.other honey en
dc.subject.other quantitative analysis en
dc.subject.other Raman spectrometry en
dc.subject.other regression analysis en
dc.subject.other reproducibility en
dc.subject.other Chromatography, High Pressure Liquid en
dc.subject.other Fructose en
dc.subject.other Glucose en
dc.subject.other Honey en
dc.subject.other Reproducibility of Results en
dc.subject.other Spectrum Analysis, Raman en
dc.title FT-Raman spectroscopic simultaneous determination of fructose and glucose in honey en
heal.type journalArticle en
heal.identifier.primary 10.1021/jf048793m en
heal.publicationDate 2005 en
heal.abstract A new method for mass percentage determination of fructose and glucose based on FT-Raman spectroscopy is evaluated with a standard HPLC-based method. FT-Raman spectra manipulation is done via the spectrometer software, and a PLS (partial least squares) method is developed with the TQ Analyst software (Ver 1. 1a). The simultaneous quantitative determination uses an input range from 1700 to 700 cm -1 without correction or baseline factors. The standards used in the PLS method are honey samples previously analyzed by HPLC to obtain their mass percentage concentrations in fructose and glucose. The returned results are statistically tested with those of the HPLC method. Both methods appear to score equally in terms of reproducibility. The honey content of the two sugars in total was found up to 40-74%. The honey samples content in fructose and glucose was determined by HPLC (24.1-42.9% and 16.2-33.1%, respectively) and FT-Raman (24.0-40.8% and 21.1-32.2%, respectively). en
heal.journalName Journal of Agricultural and Food Chemistry en
dc.identifier.issue 2 en
dc.identifier.volume 53 en
dc.identifier.doi 10.1021/jf048793m en
dc.identifier.spage 207 en
dc.identifier.epage 210 en


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