| dc.contributor.author |
Baldi, A |
en |
| dc.contributor.author |
Losio, MN |
en |
| dc.contributor.author |
Cheli, F |
en |
| dc.contributor.author |
Rebucci, R |
en |
| dc.contributor.author |
Sangalli, L |
en |
| dc.contributor.author |
Fusi, E |
en |
| dc.contributor.author |
Bertasi, B |
en |
| dc.contributor.author |
Pavoni, E |
en |
| dc.contributor.author |
Carli, S |
en |
| dc.contributor.author |
Politis, I |
en |
| dc.date.accessioned |
2014-06-06T06:46:11Z |
|
| dc.date.available |
2014-06-06T06:46:11Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
00071145 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1079/BJN20041092 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2826 |
|
| dc.subject |
Antioxidants |
en |
| dc.subject |
Cell cultures |
en |
| dc.subject |
Ochratoxin A |
en |
| dc.subject |
Reactive oxygen species |
en |
| dc.subject.other |
alpha tocopherol |
en |
| dc.subject.other |
cytochrome P450 |
en |
| dc.subject.other |
mycotoxin |
en |
| dc.subject.other |
ochratoxin |
en |
| dc.subject.other |
reactive oxygen metabolite |
en |
| dc.subject.other |
retinol |
en |
| dc.subject.other |
antioxidant |
en |
| dc.subject.other |
cytochrome P450 |
en |
| dc.subject.other |
reactive oxygen metabolite |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
antioxidant activity |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell death |
en |
| dc.subject.other |
cell line |
en |
| dc.subject.other |
cell viability |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
cytotoxicity |
en |
| dc.subject.other |
drug potentiation |
en |
| dc.subject.other |
human |
en |
| dc.subject.other |
human cell |
en |
| dc.subject.other |
LC 50 |
en |
| dc.subject.other |
lipid peroxidation |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
oxidative stress |
en |
| dc.subject.other |
statistical significance |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
cell survival |
en |
| dc.subject.other |
dose response |
en |
| dc.subject.other |
drug antagonism |
en |
| dc.subject.other |
drug effect |
en |
| dc.subject.other |
food contamination |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
Animalia |
en |
| dc.subject.other |
Bovinae |
en |
| dc.subject.other |
alpha-Tocopherol |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Antioxidants |
en |
| dc.subject.other |
Cell Line |
en |
| dc.subject.other |
Cell Survival |
en |
| dc.subject.other |
Cytochrome P-450 Enzyme System |
en |
| dc.subject.other |
Dose-Response Relationship, Drug |
en |
| dc.subject.other |
Food Contamination |
en |
| dc.subject.other |
Humans |
en |
| dc.subject.other |
Mycotoxins |
en |
| dc.subject.other |
Ochratoxins |
en |
| dc.subject.other |
Oxidative Stress |
en |
| dc.subject.other |
Reactive Oxygen Species |
en |
| dc.subject.other |
Tumor Cells, Cultured |
en |
| dc.subject.other |
Vitamin A |
en |
| dc.title |
Evaluation of the protective effects of α-toxopherol and retinol against ochratoxin A cytotoxicity |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1079/BJN20041092 |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
Ochratoxin A (OTA), a mycotoxin frequently present in food and feedstuffs, produces a wide range of toxic effects, including cell death via lipid peroxidation. In one human and four animal cell lines we determined the half lethal concentration (LC50) of OTA, its effect on reactive oxygen species (ROS) production, and its ability to induce cytochrome p450 activity. We also examined the protective effect of α-tocopherol and all-trans-retinol in the most sensitive cell lines (i.e. bovine mammary epithelia, for which LC50 was 0.8 μg/ml (24 h), and Madin Darby canine kidney, for which LC50 was 4.3 μg/ml (48 h)). Pre-incubation for 3 h with either antioxidant significantly (P<0.05) ameliorated the OTA-induced reduction in cell viability and significantly decreased (P<0.05) ROS production. These findings indicate that oxidative stress is an important factor in OTA cytotoxicity. Supplementation with antioxidant molecules may counteract the short-term toxicity of this mycotoxin. © The Authors 2004. |
en |
| heal.journalName |
British Journal of Nutrition |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
91 |
en |
| dc.identifier.doi |
10.1079/BJN20041092 |
en |
| dc.identifier.spage |
507 |
en |
| dc.identifier.epage |
512 |
en |