| dc.contributor.author |
Labrou, NE |
en |
| dc.contributor.author |
Rigden, DJ |
en |
| dc.date.accessioned |
2014-06-06T06:46:10Z |
|
| dc.date.available |
2014-06-06T06:46:10Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
00142956 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1432-1033.2004.04000.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2817 |
|
| dc.subject |
Active site |
en |
| dc.subject |
Affinity labelling |
en |
| dc.subject |
Clostripain |
en |
| dc.subject |
Molecular modelling |
en |
| dc.subject |
Peptidase family C11 |
en |
| dc.subject.other |
arginine |
en |
| dc.subject.other |
aspartic acid |
en |
| dc.subject.other |
benzamidine |
en |
| dc.subject.other |
benzoylarginine ethyl ester |
en |
| dc.subject.other |
calcium |
en |
| dc.subject.other |
caspase |
en |
| dc.subject.other |
clostripain |
en |
| dc.subject.other |
cysteine |
en |
| dc.subject.other |
diazonium compound |
en |
| dc.subject.other |
dye |
en |
| dc.subject.other |
histidine |
en |
| dc.subject.other |
mutant protein |
en |
| dc.subject.other |
peptidase |
en |
| dc.subject.other |
affinity labeling |
en |
| dc.subject.other |
architecture |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterium |
en |
| dc.subject.other |
catalysis |
en |
| dc.subject.other |
cell lineage |
en |
| dc.subject.other |
chemical reaction |
en |
| dc.subject.other |
Clostridium histolyticum |
en |
| dc.subject.other |
concentration (parameters) |
en |
| dc.subject.other |
data base |
en |
| dc.subject.other |
dissociation |
en |
| dc.subject.other |
enzyme activation |
en |
| dc.subject.other |
enzyme active site |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
enzyme assay |
en |
| dc.subject.other |
enzyme binding |
en |
| dc.subject.other |
enzyme substrate complex |
en |
| dc.subject.other |
incubation time |
en |
| dc.subject.other |
molecular model |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein domain |
en |
| dc.subject.other |
protein family |
en |
| dc.subject.other |
protein folding |
en |
| dc.subject.other |
protein function |
en |
| dc.subject.other |
protein structure |
en |
| dc.subject.other |
separation technique |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
site directed mutagenesis |
en |
| dc.subject.other |
structure activity relation |
en |
| dc.subject.other |
target organ |
en |
| dc.subject.other |
Affinity Labels |
en |
| dc.subject.other |
Amino Acid Sequence |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Bacterial Proteins |
en |
| dc.subject.other |
Binding Sites |
en |
| dc.subject.other |
Clostridium |
en |
| dc.subject.other |
Coloring Agents |
en |
| dc.subject.other |
Cysteine Endopeptidases |
en |
| dc.subject.other |
Hydrogen-Ion Concentration |
en |
| dc.subject.other |
Models, Molecular |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Mutagenesis, Site-Directed |
en |
| dc.subject.other |
Protein Conformation |
en |
| dc.subject.other |
Sequence Alignment |
en |
| dc.subject.other |
Structure-Activity Relationship |
en |
| dc.subject.other |
Substrate Specificity |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Clostridium histolyticum |
en |
| dc.title |
The structure-function relationship in the clostripain family of peptidases |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1432-1033.2004.04000.x |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
In this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. A benzamidinyldiazo dichlorotriazine dye (BDD) was shown to act as an efficient active site-directed affinity label for Clostridium histolyticum clostripain. The enzyme, upon incubation with BDD in 0.1 M Hepes/NaOH buffer pH 7.6, exhibits a time-dependent loss of activity. The rate of inactivation exhibits a nonlinear dependence on the BDD concentration, which can be described by reversible binding of dye to the enzyme prior to the irreversible reaction. The dissociation constant of the reversible formation of an enzyme-BDD complex is KD = 74.6 ± 2.1 μM and the maximal rate constant of inactivation is k3 = 0.21·min-1. Effective protection against inactivation by BDD is provided by the substrate N-benzoyl-L-arginine ethyl ester (BAEE). Cleavage of BDD-modified enzyme with trypsin and subsequent separation of peptides by reverse-phase HPLC gave only one modified peptide. Amino acid sequencing of the modified tryptic peptide revealed the target site of BDD reaction to be His176. Site-directed mutagenesis was used to study further the functional role of His176. The mutant His176A1a enzyme exhibited zero activity against BAEE. Together with previous data, these results confirm that a catalytic dyad of His176 and Cys231 is responsible for cysteine peptidase activity in the C11 peptidase family. A molecular model of the catalytic domain of clostripain was constructed using a manually extended fold recognition-derived alignment with caspases. A rigorous iterative modelling scheme resulted in an objectively sound model which points to Asp229 as responsible for defining the strong substrate specificity for Arg at the P1 position. Two possible binding sites for the calcium required for auto-activation could be located. Database searches show that clostripain homologues are not confined to bacterial lineages and reveal an intriguing variety of domain architectures. |
en |
| heal.journalName |
European Journal of Biochemistry |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
271 |
en |
| dc.identifier.doi |
10.1111/j.1432-1033.2004.04000.x |
en |
| dc.identifier.spage |
983 |
en |
| dc.identifier.epage |
992 |
en |