The galactolipid digalactosyldiacylglycerol accumulates in the peribacteroid membrane of nitrogen-fixing nodules of soybean and Lotus

dc.contributor.author	Gaude, N	en
dc.contributor.author	Tippmann, H	en
dc.contributor.author	Flemetakis, E	en
dc.contributor.author	Katinakis, P	en
dc.contributor.author	Udvardi, M	en
dc.contributor.author	Dormann, P	en
dc.date.accessioned	2014-06-06T06:46:09Z
dc.date.available	2014-06-06T06:46:09Z
dc.date.issued	2004	en
dc.identifier.issn	00219258	en
dc.identifier.uri	http://dx.doi.org/10.1074/jbc.M404098200	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2811
dc.subject.other	Bacteria	en
dc.subject.other	Composition	en
dc.subject.other	Contamination	en
dc.subject.other	Fatty acids	en
dc.subject.other	Glycerol	en
dc.subject.other	Nitrogen fixation	en
dc.subject.other	Phosphorus	en
dc.subject.other	Proteins	en
dc.subject.other	Bacteroids	en
dc.subject.other	Cortical tissues	en
dc.subject.other	Legumes	en
dc.subject.other	Perbacteroid membrane (PBM)	en
dc.subject.other	Cell membranes	en
dc.subject.other	complementary DNA	en
dc.subject.other	digalactosyldiglyceride	en
dc.subject.other	galactolipid	en
dc.subject.other	phosphate	en
dc.subject.other	synthetase	en
dc.subject.other	unclassified drug	en
dc.subject.other	Arabidopsis protein	en
dc.subject.other	DGD1 protein, Arabidopsis	en
dc.subject.other	fatty acid	en
dc.subject.other	galactosyltransferase	en
dc.subject.other	lipid	en
dc.subject.other	nucleic acid	en
dc.subject.other	Arabidopsis	en
dc.subject.other	article	en
dc.subject.other	bacterial membrane	en
dc.subject.other	DNA sequence	en
dc.subject.other	gene expression	en
dc.subject.other	lipid composition	en
dc.subject.other	lipid storage	en
dc.subject.other	lipogenesis	en
dc.subject.other	Lotus	en
dc.subject.other	membrane marker	en
dc.subject.other	nitrogen fixation	en
dc.subject.other	nodulation	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	peribacteroid membrane	en
dc.subject.other	plastid	en
dc.subject.other	priority journal	en
dc.subject.other	Rhizobiaceae	en
dc.subject.other	soybean	en
dc.subject.other	cell membrane	en
dc.subject.other	chemistry	en
dc.subject.other	in situ hybridization	en
dc.subject.other	lipid metabolism	en
dc.subject.other	metabolism	en
dc.subject.other	molecular genetics	en
dc.subject.other	Northern blotting	en
dc.subject.other	plant leaf	en
dc.subject.other	plant root	en
dc.subject.other	Western blotting	en
dc.subject.other	Arabidopsis	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Glycine max	en
dc.subject.other	Lotus	en
dc.subject.other	Lotus corniculatus var. japonicus	en
dc.subject.other	Rhizobiaceae	en
dc.subject.other	Arabidopsis Proteins	en
dc.subject.other	Blotting, Northern	en
dc.subject.other	Blotting, Western	en
dc.subject.other	Cell Membrane	en
dc.subject.other	DNA, Complementary	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Galactolipids	en
dc.subject.other	Galactosyltransferases	en
dc.subject.other	In Situ Hybridization	en
dc.subject.other	Lipid Metabolism	en
dc.subject.other	Lipids	en
dc.subject.other	Lotus	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Nucleic Acids	en
dc.subject.other	Phosphates	en
dc.subject.other	Plant Leaves	en
dc.subject.other	Plant Roots	en
dc.subject.other	Plastids	en
dc.subject.other	Soybeans	en
dc.title	The galactolipid digalactosyldiacylglycerol accumulates in the peribacteroid membrane of nitrogen-fixing nodules of soybean and Lotus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1074/jbc.M404098200	en
heal.publicationDate	2004	en
heal.abstract	The peribacteroid membrane (PBM) surrounding nitrogen fixing rhizobia in the nodules of legumes is crucial for the exchange of ammonium and nutrients between the bacteria and the host cell. Digalactosyldiacylglycerol (DGDG), a galactolipid abundant in chloroplasts, was detected in the PBM of soybean (Glycine max) and Lotus japonicus. Analyses of membrane marker proteins and of fatty acid composition confirmed that DGDG represents an authentic PBM lipid of plant origin and is not derived from the bacteria or from plastid contamination. In Arabidopsis, DGDG is known to accumulate in extraplastidic membranes during phosphate deprivation. However, the presence of DGDG in soybean PBM was not restricted to phosphate limiting conditions. Complementary DNA sequences corresponding to the two DGDG synthases, DGD1 and DGD2 from Arabidopsis, were isolated from soybean and Lotus. The two genes were expressed during later stages of nodule development in infected cells and in cortical tissue. Because nodule development depends on the presence of high amounts of phosphate in the growth medium, the accumulation of the non-phosphorus galactolipid DGDG in the PBM might be important to save phosphate for other essential processes, i.e. nucleic acid synthesis in bacteroids and host cells.	en
heal.journalName	Journal of Biological Chemistry	en
dc.identifier.issue	33	en
dc.identifier.volume	279	en
dc.identifier.doi	10.1074/jbc.M404098200	en
dc.identifier.spage	34624	en
dc.identifier.epage	34630	en