The effect of raw glycerol concentration on the production of 1,3-propanediol by Clostridium butyricum

dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Fick, M	en
dc.contributor.author	Aggelis, G	en
dc.date.accessioned	2014-06-06T06:46:09Z
dc.date.available	2014-06-06T06:46:09Z
dc.date.issued	2004	en
dc.identifier.issn	02682575	en
dc.identifier.uri	http://dx.doi.org/10.1002/jctb.1103	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2808
dc.subject	1,3-propanediol	en
dc.subject	Clostridium butyricum	en
dc.subject	Modelling	en
dc.subject	Raw glycerol	en
dc.subject.other	Acetic acid	en
dc.subject.other	Alcohols	en
dc.subject.other	Bacteria	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Biosynthesis	en
dc.subject.other	Cell culture	en
dc.subject.other	Diesel fuels	en
dc.subject.other	Metabolism	en
dc.subject.other	Metabolites	en
dc.subject.other	Anaebolic reaction	en
dc.subject.other	Bio-diesel production process	en
dc.subject.other	Constant dilution rates	en
dc.subject.other	Glycerol	en
dc.subject.other	acetic acid	en
dc.subject.other	butyric acid	en
dc.subject.other	carbon dioxide	en
dc.subject.other	carboxylic acid	en
dc.subject.other	glycerol	en
dc.subject.other	organic carbon	en
dc.subject.other	propanediol derivative	en
dc.subject.other	reduced nicotinamide adenine dinucleotide	en
dc.subject.other	biotechnology	en
dc.subject.other	article	en
dc.subject.other	bacterial cell	en
dc.subject.other	bacterial metabolism	en
dc.subject.other	bacterium culture	en
dc.subject.other	batch process	en
dc.subject.other	biomass production	en
dc.subject.other	biosynthesis	en
dc.subject.other	biotechnology	en
dc.subject.other	biotransformation	en
dc.subject.other	chemical reaction kinetics	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	controlled study	en
dc.subject.other	culture medium	en
dc.subject.other	microbial biomass	en
dc.subject.other	nonhuman	en
dc.subject.other	statistical model	en
dc.subject.other	Clostridium	en
dc.subject.other	Clostridium butyricum	en
dc.title	The effect of raw glycerol concentration on the production of 1,3-propanediol by Clostridium butyricum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/jctb.1103	en
heal.publicationDate	2004	en
heal.abstract	Raw glycerol, the main by-product of the bio-diesel production process, was converted to 1,3-propanediol by Clostridium butyricum F2b. In batch cultures, 47.1 g dm-3 of 1,3-propanediol were produced. Continuous cultures were conducted at a constant dilution rate (=0.04h-1) and various inlet glycerol concentrations with 1,3-propanediol produced at levels up to 44.0 g dm-3. At increasing glycerol concentrations in the inlet medium, biomass yield decreased. This decrease was attributed to the microbial metabolism being directed towards the biosynthesis of organic acids (and hence carbon losses as CO2) instead of biochemical anabolic reactions. An autonomous analytical model was developed, and quantified the effect of inlet glycerol concentration on the production of biomass and metabolites. Indeed, high inlet substrate concentrations positively affected the biosynthesis, principally of butyric acid and to a lesser extent that of acetic acid. In contrast, at increased glycerol concentrations, the relative increase of 1,3-propanediol production per unit of substrate consumed was lower as compared with that of acetic and, mainly, butyric acid. This could be explained by the fact that the butyric acid pathway represents an alternative and competitive one to that of 1,3-propanediol for re-generation of NADH2 equivalents in the microbial cell. © 2004 Society of Chemical Industry.	en
heal.journalName	Journal of Chemical Technology and Biotechnology	en
dc.identifier.issue	11	en
dc.identifier.volume	79	en
dc.identifier.doi	10.1002/jctb.1103	en
dc.identifier.spage	1189	en
dc.identifier.epage	1196	en