| dc.contributor.author |
Labrinea, EP |
en |
| dc.contributor.author |
Georgiou, CA |
en |
| dc.date.accessioned |
2014-06-06T06:46:08Z |
|
| dc.date.available |
2014-06-06T06:46:08Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
00032670 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.aca.2004.09.040 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2803 |
|
| dc.subject |
ABTS |
en |
| dc.subject |
Stopped flow |
en |
| dc.subject |
Total antioxidant capacity |
en |
| dc.subject.other |
Antioxidant compounds |
en |
| dc.subject.other |
Glutathione |
en |
| dc.subject.other |
Trlox equivalent antioxidant capacity |
en |
| dc.subject.other |
Antioxidants |
en |
| dc.subject.other |
Positive ions |
en |
| dc.subject.other |
Reaction kinetics |
en |
| dc.subject.other |
pH effects |
en |
| dc.subject.other |
2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid) |
en |
| dc.subject.other |
albumin |
en |
| dc.subject.other |
antioxidant |
en |
| dc.subject.other |
benzothiazoline derivative |
en |
| dc.subject.other |
butylcresol |
en |
| dc.subject.other |
catechin |
en |
| dc.subject.other |
cation |
en |
| dc.subject.other |
epicatechin |
en |
| dc.subject.other |
gallic acid |
en |
| dc.subject.other |
glutathione |
en |
| dc.subject.other |
para coumaric acid |
en |
| dc.subject.other |
quercetin |
en |
| dc.subject.other |
trolox C |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
antioxidant activity |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
assay |
en |
| dc.subject.other |
chemical structure |
en |
| dc.subject.other |
pH |
en |
| dc.subject.other |
priority journal |
en |
| dc.title |
Stopped-flow method for assessment of pH and timing effect on the ABTS total antioxidant capacity assay |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.aca.2004.09.040 |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
The pH and timing effect on the relative capacity of antioxidant compounds to scavenge the ABTS [2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)] radical cation (ABTS •+) expressed as the trolox equivalent antioxidant capacity (TEAC) is assessed for a wide range of antioxidants using a modification of the original ABTS assay at different pHs (4.6, 5.4 and 7.4). To study both fast and slow reacting antioxidants, a stopped-flow method based on a low cost laboratory-made system was used. For most of the tested antioxidants, total antioxidant capacity values were found to be a function of pH and time allowed for the reaction. Structurally similar compounds have the same time and pH-dependent behaviour even if they have significant differences in total antioxidant capacity values. The ABTS •+ scavenging reaction rate was found to depend strongly on pH for p-coumaric acid, glutathione, BHT and albumin. Quercetin, gallic acid, (+)-catechin and (-)-epicatechin showed the higher activities at all pHs. The stopped-flow method can be utilized for screening of antioxidant compounds of unknown kinetics towards ABTS •+ at different pHs and the results can be used to predict the total antioxidant capacity of structurally related compounds. © 2004 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Analytica Chimica Acta |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
526 |
en |
| dc.identifier.doi |
10.1016/j.aca.2004.09.040 |
en |
| dc.identifier.spage |
63 |
en |
| dc.identifier.epage |
68 |
en |