In vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several Ophrys species (Orchidaceae)

dc.contributor.author	Kitsaki, CK	en
dc.contributor.author	Zygouraki, S	en
dc.contributor.author	Ziobora, M	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:46:01Z
dc.date.available	2014-06-06T06:46:01Z
dc.date.issued	2004	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00299-004-0841-8	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2759
dc.subject	Callogenesis	en
dc.subject	Immature seed	en
dc.subject	In vitro germination	en
dc.subject	Mature seed	en
dc.subject	Ophrys	en
dc.subject.other	Genes	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Seed	en
dc.subject.other	Minitubers	en
dc.subject.other	Protocorm formation	en
dc.subject.other	Seed maturity	en
dc.subject.other	Plants (botany)	en
dc.subject.other	article	en
dc.subject.other	culture medium	en
dc.subject.other	culture technique	en
dc.subject.other	genetics	en
dc.subject.other	genotype	en
dc.subject.other	germination	en
dc.subject.other	Greece	en
dc.subject.other	growth, development and aging	en
dc.subject.other	in vitro study	en
dc.subject.other	methodology	en
dc.subject.other	Orchidaceae	en
dc.subject.other	physiology	en
dc.subject.other	plant seed	en
dc.subject.other	Culture Media	en
dc.subject.other	Culture Techniques	en
dc.subject.other	Genotype	en
dc.subject.other	Germination	en
dc.subject.other	Greece	en
dc.subject.other	Orchidaceae	en
dc.subject.other	Seeds	en
dc.subject.other	Genotypes	en
dc.subject.other	Germination	en
dc.subject.other	Plants	en
dc.subject.other	Seeds	en
dc.subject.other	Tubers	en
dc.subject.other	Ananas comosus	en
dc.subject.other	Cornuta	en
dc.subject.other	Ophrys	en
dc.subject.other	Ophrys apifera	en
dc.subject.other	Ophrys argolica	en
dc.subject.other	Ophrys attica	en
dc.subject.other	Ophrys cornuta	en
dc.subject.other	Ophrys delphinensis	en
dc.subject.other	Ophrys ferrum-equinum	en
dc.subject.other	Ophrys mammosa	en
dc.subject.other	Ophrys spruneri	en
dc.subject.other	Ophrys tenthredinifera	en
dc.subject.other	Orchidaceae	en
dc.title	In vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several Ophrys species (Orchidaceae)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00299-004-0841-8	en
heal.publicationDate	2004	en
heal.abstract	We investigated the effect of genotype, seed maturity and culture medium on the in vitro germination and development of protocorms and plantlets from seeds of 13 different Ophrys species (O. apifera, O. attica, O. cornuta, O. delfinensis, O. ferrum-equinum, O. lutea, O. mammosa, O. speculum, O. spruneri, O. umbilicata, O. argolica, O. irricolor and O. tenthredinifera) collected in Greece, some of which are endemic to this country. Mature seeds (10 months after collection) and immature seeds (2 months after anthesis) were cultured in a coconut milk-enriched or a pineapple-enriched medium (CEM or PEM, respectively). The highest percentage of callogenesis (96%) was observed in immature seeds of O. delphinensis in the CEM, while the highest percentage of protocorm formation (52%) was observed in mature seeds of O. spuneri in the CEM. Protocorm formation was significantly lower in immature seeds than in mature seeds in both culture media. Eventually almost all of the transferred protocorms developed to plantlets, which later formed minitubers. PEM appeared to be the most suitable for the development of minitubers from plantlets. All of the factors investigated - as well as their interactions - significantly affected callogenesis and protocorm formation. The results are discussed with the perspective of applying an improved protocol for in vitro seed germination and plantlet formation in several under-utilized Ophrys species. © Springer-Verlag 2004.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	5	en
dc.identifier.volume	23	en
dc.identifier.doi	10.1007/s00299-004-0841-8	en
dc.identifier.spage	284	en
dc.identifier.epage	290	en