| dc.contributor.author |
Politis, I |
en |
| dc.contributor.author |
Bizelis, I |
en |
| dc.contributor.author |
Tsiaras, A |
en |
| dc.contributor.author |
Baldi, A |
en |
| dc.date.accessioned |
2014-06-06T06:45:58Z |
|
| dc.date.available |
2014-06-06T06:45:58Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
00220299 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1017/S002202990400010X |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2736 |
|
| dc.subject |
Dairy cows |
en |
| dc.subject |
Neutrophil function |
en |
| dc.subject |
Plasmin activity |
en |
| dc.subject |
Vitamin E |
en |
| dc.subject.other |
alpha tocopherol |
en |
| dc.subject.other |
lactose |
en |
| dc.subject.other |
lipid |
en |
| dc.subject.other |
milk protein |
en |
| dc.subject.other |
phorbol 13 acetate 12 myristate |
en |
| dc.subject.other |
plasmin |
en |
| dc.subject.other |
plasminogen |
en |
| dc.subject.other |
superoxide |
en |
| dc.subject.other |
urokinase |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cattle |
en |
| dc.subject.other |
cell count |
en |
| dc.subject.other |
chemistry |
en |
| dc.subject.other |
cytology |
en |
| dc.subject.other |
diet supplementation |
en |
| dc.subject.other |
enzymology |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
milk |
en |
| dc.subject.other |
neutrophil |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
pregnancy |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cattle |
en |
| dc.subject.other |
Cell Count |
en |
| dc.subject.other |
Dietary Supplements |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Lactose |
en |
| dc.subject.other |
Lipids |
en |
| dc.subject.other |
Milk |
en |
| dc.subject.other |
Milk Proteins |
en |
| dc.subject.other |
Neutrophils |
en |
| dc.subject.other |
Plasmin |
en |
| dc.subject.other |
Plasminogen |
en |
| dc.subject.other |
Pregnancy |
en |
| dc.subject.other |
Superoxides |
en |
| dc.subject.other |
Tetradecanoylphorbol Acetate |
en |
| dc.subject.other |
Urinary Plasminogen Activator |
en |
| dc.subject.other |
Vitamin E |
en |
| dc.subject.other |
Animalia |
en |
| dc.subject.other |
Bos taurus |
en |
| dc.subject.other |
Bovinae |
en |
| dc.title |
Effect of vitamin E supplementation on neutrophil function, milk composition and plasmin activity in dairy cows in a commercial herd |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1017/S002202990400010X |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
Fifty-six Holstein dairy cows from a commercial dairy herd in the Northern part of Greece were used to determine the effect of vitamin E supplementation on immune parameters, milk composition and milk quality. Cows were assigned to one of two experimental groups: control (no vitamin E supplementation) and vitamin E supplementation. Supplementation of vitamin E started 4 weeks prior to and continued up to 12 weeks after parturition. Supplementation included daily oral administration of vitamin E at 3000 i.u./cow prepartum and was reduced to 1000 i.u./cow post partum. Blood samples were collected weekly for 8 weeks starting 4 weeks before parturition, neutrophils were isolated and the following parameters were determined in neutrophils activated by phorbol myristate acetate: total cell-associated and membrane-bound urokinase plasminogen activator (u-PA) activity and superoxide production. Milk samples were collected weekly and fat, protein, lactose, somatic cell count (SCC), plasmin and plasminogen-derived activity were determined. Activated neutrophils isolated from cows that received supplemental vitamin E had higher (P<0.01) total and membrane-bound u-PA activities during the first 3 weeks after parturition and higher (P<0.01) superoxide production during week 1 prepartum and week 1 post partum compared with the corresponding values of activated neutrophils isolated from control cows. Vitamin E supplementation had no effect (P=0.28) on plasminogen-derived activity in milk. Milk obtained from cows that received supplemental vitamin E had SCC lower by 25% (P<0.05) and plasmin lower by 30% (P<0.01) than corresponding values in milk obtained from control cows. The reduction in plasmin as a result of vitamin E supplementation is very beneficial to the dairy industry because plasmin reduces the cheese-yielding capacity of milk, affects the coagulating properties of milk and its overall ability to withstand processing during cheesemaking. In conclusion, vitamin E supplementation had positive effects on the function of bovine neutrophils and milk quality in a commercial dairy herd. |
en |
| heal.journalName |
Journal of Dairy Research |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
71 |
en |
| dc.identifier.doi |
10.1017/S002202990400010X |
en |
| dc.identifier.spage |
273 |
en |
| dc.identifier.epage |
278 |
en |