| dc.contributor.author |
Hopkins, L |
en |
| dc.contributor.author |
Parmar, S |
en |
| dc.contributor.author |
Bouranis, DL |
en |
| dc.contributor.author |
Howarth, JR |
en |
| dc.contributor.author |
Hawkesford, MJ |
en |
| dc.date.accessioned |
2014-06-06T06:45:56Z |
|
| dc.date.available |
2014-06-06T06:45:56Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
14358603 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1055/s-2004-820872 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2717 |
|
| dc.subject |
APS-reductase |
en |
| dc.subject |
ATP-sulfurylase |
en |
| dc.subject |
Maize |
en |
| dc.subject |
Root morphology |
en |
| dc.subject |
Sulfate transporter |
en |
| dc.subject |
Sulfur assimilation |
en |
| dc.subject.other |
adenylylsulfate reductase |
en |
| dc.subject.other |
carrier protein |
en |
| dc.subject.other |
complementary DNA |
en |
| dc.subject.other |
oxidoreductase |
en |
| dc.subject.other |
plant DNA |
en |
| dc.subject.other |
SLC26A4 protein, human |
en |
| dc.subject.other |
sulfate |
en |
| dc.subject.other |
sulfate adenylyltransferase |
en |
| dc.subject.other |
vegetable protein |
en |
| dc.subject.other |
maize |
en |
| dc.subject.other |
nutrient availability |
en |
| dc.subject.other |
nutrient uptake |
en |
| dc.subject.other |
root system |
en |
| dc.subject.other |
root-shoot ratio |
en |
| dc.subject.other |
starvation |
en |
| dc.subject.other |
sulfate |
en |
| dc.subject.other |
active transport |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
in situ hybridization |
en |
| dc.subject.other |
maize |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
molecular cloning |
en |
| dc.subject.other |
molecular genetics |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
phylogeny |
en |
| dc.subject.other |
plant root |
en |
| dc.subject.other |
reverse transcription polymerase chain reaction |
en |
| dc.subject.other |
Base Sequence |
en |
| dc.subject.other |
Biological Transport, Active |
en |
| dc.subject.other |
Carrier Proteins |
en |
| dc.subject.other |
Cloning, Molecular |
en |
| dc.subject.other |
DNA, Complementary |
en |
| dc.subject.other |
DNA, Plant |
en |
| dc.subject.other |
In Situ Hybridization |
en |
| dc.subject.other |
Membrane Transport Proteins |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Oxidoreductases Acting on Sulfur Group Donors |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Plant Proteins |
en |
| dc.subject.other |
Plant Roots |
en |
| dc.subject.other |
Reverse Transcriptase Polymerase Chain Reaction |
en |
| dc.subject.other |
Sulfate Adenylyltransferase |
en |
| dc.subject.other |
Sulfates |
en |
| dc.subject.other |
Zea mays |
en |
| dc.subject.other |
Zea |
en |
| dc.subject.other |
Zea mays |
en |
| dc.title |
Coordinated expression of sulfate uptake and components of the sulfate assimilatory pathway in maize |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1055/s-2004-820872 |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
A high-affinity-type sulfate transporter (Group 1: ZmST1;1, Accession No. AF355602) has been cloned from maize seedlings by RT-PCR. Tissue and cell specific localisation of this sulfate transporter has been determined along the developmental gradient of the root and in leaves of different ages. In S-sufficient conditions there was uniform low expression of ZmST1;1 in the root and very low expression in the leaves. Increased mRNA abundance and sulfate influx capacity indicated that S-starvation increased ZmST1;1 expression in roots, especially at the top of the root (just behind the seed, the area possessing most laterals and root hairs) compared to the root tip. Similarly a group 2, probable low affinity-type sulfate transporter, ZmST2;1, and also ATP-sulfurylase and APS-reductase but not OAS(thiol)lyase were induced by S-starvation and showed highest expression in the upper section of the root. S-starvation increased root/shoot ratio by 20% and increased root lateral length and abundance in the region closest to the root tip. As the increase in root proliferation was not as great as the increase in mRNA pools, it was clear that there was a higher cellular abundance of the mRNAs for sulfate transporters, ATP-sulfurylase, and APS-reductase in response to sulfur starvation. In the leaves, the sulfate transporters, ATP-sulfurylase and APS-reductase were induced by S-starvation with the most mature leaf showing increased mRNA abundance first. In situ hybridization indicated that ZmST1;1 was expressed in epidermal and endodermal cell layers throughout the root whilst OAS(thiol)lyase was highly expressed in the root cortex. |
en |
| heal.journalName |
Plant Biology |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
6 |
en |
| dc.identifier.doi |
10.1055/s-2004-820872 |
en |
| dc.identifier.spage |
408 |
en |
| dc.identifier.epage |
414 |
en |