| dc.contributor.author |
Moatsou, G |
en |
| dc.contributor.author |
Samolada, M |
en |
| dc.contributor.author |
Katsabeki, A |
en |
| dc.contributor.author |
Anifantakis, E |
en |
| dc.date.accessioned |
2014-06-06T06:45:55Z |
|
| dc.date.available |
2014-06-06T06:45:55Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
00237302 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1051/lait:2004006 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2707 |
|
| dc.subject |
Chromatography |
en |
| dc.subject |
Electrophoresis |
en |
| dc.subject |
Indigenous ovine breeds |
en |
| dc.subject |
Ovine casein |
en |
| dc.subject |
RP-HPLC |
en |
| dc.subject.other |
Distillation columns |
en |
| dc.subject.other |
Electrophoresis |
en |
| dc.subject.other |
Fractionation |
en |
| dc.subject.other |
High performance liquid chromatography |
en |
| dc.subject.other |
Ion exchange |
en |
| dc.subject.other |
Statistical methods |
en |
| dc.subject.other |
Urea |
en |
| dc.subject.other |
Indigeneous ovine breeds |
en |
| dc.subject.other |
Ovine casein |
en |
| dc.subject.other |
Ovine milk |
en |
| dc.subject.other |
Reverse phase chromatography (RP) |
en |
| dc.subject.other |
Casein |
en |
| dc.subject.other |
Ovis |
en |
| dc.title |
Casein fraction of ovine milk from indigenous Greek breeds |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1051/lait:2004006 |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
Whole casein fractions isoelectrically prepared from bulk milks from four different indigenous Greek ovine breeds were analysed by urea-PAGE and reversed-phase HPLC. Individual caseins prepared by fractionation on a cation-exchange column were used to locate the peaks on the chromatograms. Apart from heterogeneity that was depicted in the peak shape of caseins, there was also quantitative variability regarding the α s- and β-casein contents. According to the RP-HPLC results, the relative percentages of individual caseins ranged as follows: κ-casein from 9.1% to 10.8%, α s2-casein from 12.0% to 16.4%, α s1- casein from 33.9% to 39.9% and β-casein from 37.0% to 42.3%. According to the results obtained by both methods, the composition of the casein fraction from the Karagouniko breed was the most different, since it had the greatest β-casein content and the lowest α s-casein to β-casein ratio. The relative percentages of the individual caseins in two of the four breeds (Frisarta and Chios) were similar. The quantification results of urea-PAGE and RP-HPLC analyses were in general similar with respect to the means. However, the relative standard deviations of the chromatographic method were better and this was also true for the resolution of the components of the whole casein fraction. |
en |
| heal.journalName |
Lait |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
84 |
en |
| dc.identifier.doi |
10.1051/lait:2004006 |
en |
| dc.identifier.spage |
285 |
en |
| dc.identifier.epage |
296 |
en |