| dc.contributor.author |
Kintzios, S |
en |
| dc.contributor.author |
Stavropoulou, E |
en |
| dc.contributor.author |
Skamneli, S |
en |
| dc.date.accessioned |
2014-06-06T06:45:54Z |
|
| dc.date.available |
2014-06-06T06:45:54Z |
|
| dc.date.issued |
2004 |
en |
| dc.identifier.issn |
01689452 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.plantsci.2004.05.021 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2696 |
|
| dc.subject |
Cucumis melo L. |
en |
| dc.subject |
Genotypic effect |
en |
| dc.subject |
Mineral macronutrients |
en |
| dc.subject |
Organogenesis |
en |
| dc.subject |
Somatic embryogenesis |
en |
| dc.subject.other |
Biosynthesis |
en |
| dc.subject.other |
Carbohydrates |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
Ions |
en |
| dc.subject.other |
Metabolism |
en |
| dc.subject.other |
Cotyledonal plants |
en |
| dc.subject.other |
Macronutrients |
en |
| dc.subject.other |
Plants (botany) |
en |
| dc.subject.other |
Cucumis |
en |
| dc.subject.other |
Cucumis melo |
en |
| dc.title |
Accumulation of selected macronutrients and carbohydrates in melon tissue cultures: Association with pathways of in vitro dedifferentiation and differentiation (organogenesis, somatic embryogenesis) |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.plantsci.2004.05.021 |
en |
| heal.publicationDate |
2004 |
en |
| heal.abstract |
We investigated the association between the accumulation of selected macronutrient ions and soluble carbohydrates and the in vitro developmental fate of cotyledonal explants from two melon (Cucumis melo L.) cultivars. The accumulation of all macronutrients was very significantly affected by the pattern of ontogenic development, genotype and their interaction. In both cultivars, a lower NO3- accumulation was observed in callus tissues, compared to differentiated embryogenic or organogenic cultures, while direct adventitious organogenesis was associated with a considerable increase in PO43- concentration. The accumulation of K+ depended on culture development in the following order: callus induction > somatic embryogenesis > adventitious organogenesis. The highest Mg2+ concentration was associated with somatic embryogenesis and the lowest Mg2+ and Fe3+ concentrations were associated with callus induction. When considering the developmental process-specific accumulation over both genotypes, callus induction was associated with increased Ca2+, and carbohydrate concentrations and adventitious organogenesis was associated with increased NH4+ and NO3- concentrations. In vitro cultures from both cultivars accumulated more PO43- and K +, but less Mg2+ than donor plants. Embryogenic cultures from one cultivar (Bolero) accumulated two-fold more NH4+ and more than 21-fold NO3- than donor plants. However, the observed macronutrient concentrations in both cultivars in vitro were considerably lower than in the original Murashige and Skoog basal medium formulation. Results are discussed in view of the possible metabolic pathways requiring the studied macronutrients, as well as improving medium formulations and culture protocols for the micropropagation of this commercially important species. © 2004 Published by Elsevier Ireland Ltd. |
en |
| heal.journalName |
Plant Science |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
167 |
en |
| dc.identifier.doi |
10.1016/j.plantsci.2004.05.021 |
en |
| dc.identifier.spage |
655 |
en |
| dc.identifier.epage |
664 |
en |