| dc.contributor.author |
Politis, I |
en |
| dc.contributor.author |
Voudouri, A |
en |
| dc.contributor.author |
Bizelis, I |
en |
| dc.contributor.author |
Zervas, G |
en |
| dc.date.accessioned |
2014-06-06T06:45:37Z |
|
| dc.date.available |
2014-06-06T06:45:37Z |
|
| dc.date.issued |
2003 |
en |
| dc.identifier.issn |
00071145 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1079/BJN2002767 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2546 |
|
| dc.subject |
α-Tocopherol |
en |
| dc.subject |
Protein kinase C |
en |
| dc.subject |
Urokinase-plasminogen activator |
en |
| dc.subject.other |
1 (5 isoquinolinesulfonyl) 2 methylpiperazine |
en |
| dc.subject.other |
alpha tocopherol |
en |
| dc.subject.other |
alpha tocopherol derivative |
en |
| dc.subject.other |
alpha tocopherol succinate |
en |
| dc.subject.other |
n (2 guanidinoethyl) 5 isoquinolinesulfonamide |
en |
| dc.subject.other |
phorbol 13 acetate 12 myristate |
en |
| dc.subject.other |
phorbol didecanoate |
en |
| dc.subject.other |
protein kinase C |
en |
| dc.subject.other |
protein kinase C inhibitor |
en |
| dc.subject.other |
urokinase |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding kinetics |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
cell stimulation |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
in vitro study |
en |
| dc.subject.other |
macrophage |
en |
| dc.subject.other |
neutrophil |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
protein induction |
en |
| dc.subject.other |
sheep |
en |
| dc.subject.other |
vitamin supplementation |
en |
| dc.subject.other |
alpha-Tocopherol |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Cyclic AMP-Dependent Protein Kinases |
en |
| dc.subject.other |
Enzyme Inhibitors |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Macrophage Activation |
en |
| dc.subject.other |
Macrophages |
en |
| dc.subject.other |
Neutrophil Activation |
en |
| dc.subject.other |
Neutrophils |
en |
| dc.subject.other |
Protein Kinase C |
en |
| dc.subject.other |
Sheep |
en |
| dc.subject.other |
Tetradecanoylphorbol Acetate |
en |
| dc.subject.other |
Urinary Plasminogen Activator |
en |
| dc.subject.other |
Vitamin E |
en |
| dc.subject.other |
Animalia |
en |
| dc.subject.other |
Ovis |
en |
| dc.subject.other |
Ovis aries |
en |
| dc.title |
The effect of various vitamin E derivatives on the urokinase-plasminogen activator system of ovine macrophages and neutrophils |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1079/BJN2002767 |
en |
| heal.publicationDate |
2003 |
en |
| heal.abstract |
The effect of vitamin E derivatives on the urokinase-plasminogen activator (u-PA) system of resting and phorbol myristate acetate (PMA)-activated ovine macrophages and neutrophils were investigated. Blood monocyte-macrophages and neutrophils were isolated from twenty-four animals. Macrophages or neutrophils were cultured in vitro for 3 or 24h with or without various vitamin E derivatives: free α-tocopherol (α-T), α-tocopheryl acetate (α-TA), or α-tocopheryl succinate (α-TS). Following incubation, cells were stimulated with 80 μM-PMA. Total cell-associated u-PA, membrane-bound u-PA and free u-PA binding sites were determined before and after stimulation with PMA. Results showed that none of the vitamin E derivatives had any effect (P>0.05) on the u-PA system of resting monocyte-macrophages or neutrophils. In contrast, α-TS, but not α-TA or α-T, increased (P<0.01) total cell-associated u-PA and membrane-bound u-PA of PMA-stimulated macrophages and neutrophils. α-TS had no effect (P>0.05) on total u-PA and membrane-bound u-PA activities of macrophages and neutrophils cultured in the presence of 4-phorbol 12,13 didecanoate, a phorbol ester that does not activate protein kinase (PK) C. Addition of H7 (1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride), which is a potent inhibitor of both PK A and C, completely abolished the effect of α-TS on total cell-associated u-PA and membrane-bound u-PA of PMA-activated macrophages and neutrophils. Addition of HA1004 (N-(2-quanidinoethyl)-5-isoquinoline sulfonamide hydrochloride), which is a potent PK A but a weak PK C inhibitor, had no effect (P>0.05) on total cell-associated u-PA and membrane-bound u-PA of PMA-activated macrophages and neutrophils cultured in the presence of α-TS. Thus, PK C modulates the effect of α-TS on the u-PA system of ovine macrophages and neutrophils. |
en |
| heal.journalName |
British Journal of Nutrition |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
89 |
en |
| dc.identifier.doi |
10.1079/BJN2002767 |
en |
| dc.identifier.spage |
259 |
en |
| dc.identifier.epage |
265 |
en |