Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Papadelli, M en
dc.contributor.author Manolopoulou, E en
dc.contributor.author Kalantzopoulos, G en
dc.contributor.author Tsakalidou, E en
dc.date.accessioned 2014-06-06T06:45:35Z
dc.date.available 2014-06-06T06:45:35Z
dc.date.issued 2003 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/S0168-1605(02)00243-X en
dc.subject Probe en
dc.subject Species-specific PCR en
dc.subject Streptococcus macedonicus en
dc.subject.other bacterial DNA en
dc.subject.other RNA 16S en
dc.subject.other small subunit ribosomal RNA en
dc.subject.other article en
dc.subject.other bacterial strain en
dc.subject.other bacterium colony en
dc.subject.other bacterium detection en
dc.subject.other bacterium identification en
dc.subject.other bacterium isolate en
dc.subject.other DNA hybridization en
dc.subject.other lactic acid bacterium en
dc.subject.other molecular phylogeny en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other polymerase chain reaction en
dc.subject.other sensitivity and specificity en
dc.subject.other sequence analysis en
dc.subject.other species comparison en
dc.subject.other species difference en
dc.subject.other Streptococcus en
dc.subject.other Streptococcus macedonicus en
dc.subject.other Streptococcus waius en
dc.subject.other Bacterial Typing Techniques en
dc.subject.other DNA Primers en
dc.subject.other DNA, Bacterial en
dc.subject.other Molecular Sequence Data en
dc.subject.other Nucleic Acid Hybridization en
dc.subject.other Phylogeny en
dc.subject.other Polymerase Chain Reaction en
dc.subject.other RNA, Bacterial en
dc.subject.other RNA, Ribosomal, 16S en
dc.subject.other Sequence Homology, Nucleic Acid en
dc.subject.other Species Specificity en
dc.subject.other Streptococcus en
dc.subject.other Time Factors en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Streptococcus en
dc.subject.other Streptococcus macedonicus en
dc.title Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0168-1605(02)00243-X en
heal.publicationDate 2003 en
heal.abstract The aim of this study was to develop a simple and specific method for the rapid detection and identification of Streptococcus macedonicus. The method was based on polymerase chain reaction (PCR) using species-specific primers derived from the 16S rRNA gene. Specific identification was proven on seven S. macedonicus strains, while 16 strains belonging to different lactic acid bacteria species were tested negative. The PCR assay was capable of detecting 100 pg of S. macedonicus DNA, and it was also efficient on single colonies of the bacterium. Furthermore, the same bacterial strains were used for the specificity evaluation of a S. macedonicus species-specific probe. Neither species-specific PCR nor DNA hybridisation experiments could differentiate Streptococcus waius from S. macedonicus, due to the identity of the 16S rRNA gene of the two species, indicating high phylogenetical relatedness. This was further confirmed by the comparative sequence analysis of the 16S-23S rRNA intergenic regions. It was thus clearly demonstrated that S. waius, recently described as a novel Streptococcus species, is phylogenetically identical to S. macedonicus. © 2002 Elsevier Science B.V. All rights reserved. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 3 en
dc.identifier.volume 81 en
dc.identifier.doi 10.1016/S0168-1605(02)00243-X en
dc.identifier.spage 231 en
dc.identifier.epage 239 en

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account