| dc.contributor.author |
Papadelli, M |
en |
| dc.contributor.author |
Manolopoulou, E |
en |
| dc.contributor.author |
Kalantzopoulos, G |
en |
| dc.contributor.author |
Tsakalidou, E |
en |
| dc.date.accessioned |
2014-06-06T06:45:35Z |
|
| dc.date.available |
2014-06-06T06:45:35Z |
|
| dc.date.issued |
2003 |
en |
| dc.identifier.issn |
01681605 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/S0168-1605(02)00243-X |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2527 |
|
| dc.subject |
Probe |
en |
| dc.subject |
Species-specific PCR |
en |
| dc.subject |
Streptococcus macedonicus |
en |
| dc.subject.other |
bacterial DNA |
en |
| dc.subject.other |
RNA 16S |
en |
| dc.subject.other |
small subunit ribosomal RNA |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterial strain |
en |
| dc.subject.other |
bacterium colony |
en |
| dc.subject.other |
bacterium detection |
en |
| dc.subject.other |
bacterium identification |
en |
| dc.subject.other |
bacterium isolate |
en |
| dc.subject.other |
DNA hybridization |
en |
| dc.subject.other |
lactic acid bacterium |
en |
| dc.subject.other |
molecular phylogeny |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
polymerase chain reaction |
en |
| dc.subject.other |
sensitivity and specificity |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
species comparison |
en |
| dc.subject.other |
species difference |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Streptococcus macedonicus |
en |
| dc.subject.other |
Streptococcus waius |
en |
| dc.subject.other |
Bacterial Typing Techniques |
en |
| dc.subject.other |
DNA Primers |
en |
| dc.subject.other |
DNA, Bacterial |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Nucleic Acid Hybridization |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Polymerase Chain Reaction |
en |
| dc.subject.other |
RNA, Bacterial |
en |
| dc.subject.other |
RNA, Ribosomal, 16S |
en |
| dc.subject.other |
Sequence Homology, Nucleic Acid |
en |
| dc.subject.other |
Species Specificity |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Time Factors |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Streptococcus macedonicus |
en |
| dc.title |
Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/S0168-1605(02)00243-X |
en |
| heal.publicationDate |
2003 |
en |
| heal.abstract |
The aim of this study was to develop a simple and specific method for the rapid detection and identification of Streptococcus macedonicus. The method was based on polymerase chain reaction (PCR) using species-specific primers derived from the 16S rRNA gene. Specific identification was proven on seven S. macedonicus strains, while 16 strains belonging to different lactic acid bacteria species were tested negative. The PCR assay was capable of detecting 100 pg of S. macedonicus DNA, and it was also efficient on single colonies of the bacterium. Furthermore, the same bacterial strains were used for the specificity evaluation of a S. macedonicus species-specific probe. Neither species-specific PCR nor DNA hybridisation experiments could differentiate Streptococcus waius from S. macedonicus, due to the identity of the 16S rRNA gene of the two species, indicating high phylogenetical relatedness. This was further confirmed by the comparative sequence analysis of the 16S-23S rRNA intergenic regions. It was thus clearly demonstrated that S. waius, recently described as a novel Streptococcus species, is phylogenetically identical to S. macedonicus. © 2002 Elsevier Science B.V. All rights reserved. |
en |
| heal.journalName |
International Journal of Food Microbiology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
81 |
en |
| dc.identifier.doi |
10.1016/S0168-1605(02)00243-X |
en |
| dc.identifier.spage |
231 |
en |
| dc.identifier.epage |
239 |
en |