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Nitrogenous fractions during the manufacture of whey protein concentrates from Feta cheese whey

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dc.contributor.author Moatsou, G en
dc.contributor.author Hatzinaki, A en
dc.contributor.author Kandarakis, I en
dc.contributor.author Anifantakis, E en
dc.date.accessioned 2014-06-06T06:45:35Z
dc.date.available 2014-06-06T06:45:35Z
dc.date.issued 2003 en
dc.identifier.issn 03088146 en
dc.identifier.uri http://dx.doi.org/10.1016/S0308-8146(02)00414-4 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2515
dc.subject Feta cheese whey en
dc.subject Feta whey protein concentrate (WPC) en
dc.subject Nitrogenous fractions en
dc.subject Whey protein determination en
dc.subject.other nitrogen en
dc.subject.other peptone en
dc.subject.other protein en
dc.subject.other article en
dc.subject.other cheese en
dc.subject.other evaporation en
dc.subject.other food analysis en
dc.subject.other gel permeation chromatography en
dc.subject.other protein content en
dc.subject.other reversed phase high performance liquid chromatography en
dc.subject.other spray drying en
dc.subject.other statistical significance en
dc.subject.other ultrafiltration en
dc.title Nitrogenous fractions during the manufacture of whey protein concentrates from Feta cheese whey en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0308-8146(02)00414-4 en
heal.publicationDate 2003 en
heal.abstract Samples taken from different manufacturing stages of Feta cheese whey protein concentrates (WPCs), with 65 and 35% total protein content, were analyzed by chemical methods and by reversed-phase HPLC and by size-exclusion chromatography. Mean total protein content of Feta cheese whey was 1.37%. 22% of total nitrogen (TN) was non-protein N (NPN) and 12% was proteose-peptone N (PPN). The mean native N (NN), NPN and PPN percentages of WPC 65 and 35%, were 63 and 71%, 10 and 14%, 0.08 and 0.08%, respectively. According to reversed-phase HPLC data, the percentages of β-Lg and α-La and their ratio were high; 56.5 and 59% of native proteins of whey and of WPC powders, respectively, were β-Lg. The respective α-La percentages were 13.1 and 14.1% and the respective caseino-macropeptide (CMP) percentages were 12.9 and 15.3%. Apart from ultrafiltration, the most critical stage for nitrogenous fraction composition was the evaporation process before spray drying. © 2002 Elsevier Science Ltd. All rights reserved. en
heal.journalName Food Chemistry en
dc.identifier.issue 2 en
dc.identifier.volume 81 en
dc.identifier.doi 10.1016/S0308-8146(02)00414-4 en
dc.identifier.spage 209 en
dc.identifier.epage 217 en


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