| dc.contributor.author |
Tsagou, V |
en |
| dc.contributor.author |
Kefalogianni, I |
en |
| dc.contributor.author |
Sini, K |
en |
| dc.contributor.author |
Aggelis, G |
en |
| dc.date.accessioned |
2014-06-06T06:45:33Z |
|
| dc.date.available |
2014-06-06T06:45:33Z |
|
| dc.date.issued |
2003 |
en |
| dc.identifier.issn |
01757598 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s00253-003-1349-9 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/2503 |
|
| dc.subject.other |
ammonia |
en |
| dc.subject.other |
ammonium chloride |
en |
| dc.subject.other |
carbon |
en |
| dc.subject.other |
citrate synthase |
en |
| dc.subject.other |
dissolved oxygen |
en |
| dc.subject.other |
glutamate dehydrogenase |
en |
| dc.subject.other |
glutamate synthase |
en |
| dc.subject.other |
isocitrate dehydrogenase |
en |
| dc.subject.other |
ammonium |
en |
| dc.subject.other |
inhibition |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
soil microorganism |
en |
| dc.subject.other |
agriculture |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Azospirillum lipoferum |
en |
| dc.subject.other |
bacterial growth |
en |
| dc.subject.other |
bacterial metabolism |
en |
| dc.subject.other |
cultivated land |
en |
| dc.subject.other |
culture medium |
en |
| dc.subject.other |
energy resource |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
industrial waste |
en |
| dc.subject.other |
nitrogen fixation |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
Ammonium Chloride |
en |
| dc.subject.other |
Azospirillum lipoferum |
en |
| dc.subject.other |
Bacterial Proteins |
en |
| dc.subject.other |
Citrate (si)-Synthase |
en |
| dc.subject.other |
Culture Media |
en |
| dc.subject.other |
Glutamate Dehydrogenase |
en |
| dc.subject.other |
Glutamate Synthase |
en |
| dc.subject.other |
Growth Inhibitors |
en |
| dc.subject.other |
Hydroxybutyrates |
en |
| dc.subject.other |
Isocitrate Dehydrogenase |
en |
| dc.subject.other |
Oxygen |
en |
| dc.subject.other |
Polyesters |
en |
| dc.subject.other |
Polysaccharides, Bacterial |
en |
| dc.subject.other |
Soil Microbiology |
en |
| dc.subject.other |
Succinate Dehydrogenase |
en |
| dc.subject.other |
Azospirillum |
en |
| dc.subject.other |
Azospirillum lipoferum |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
cellular organisms |
en |
| dc.title |
Metabolic activities in Azospirillum lipoferum grown in the presence of NH4 + |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s00253-003-1349-9 |
en |
| heal.publicationDate |
2003 |
en |
| heal.abstract |
The utilization of some agro-industrial wastes as soil conditioners to provide free-living nitrogen-fixing bacterial populations (e.g. Azospirillum spp.) with carbon and energy sources, may be an interesting perspective for agriculture. However, the presence of ammonium nitrogen in cultivated soils and/or various wastes could inhibit the growth of the nitrogen-fixing populations. The present investigation shows that growth of Azospirillum lipoferum was restricted at a dissolved oxygen (DO) concentration equal to 135 μM, when the initial NH4Cl concentration increased from 0.5 to 0. 9 g/l. The activities of both citrate synthase (CS) and isocitrate dehydrogenase were significantly decreased in the presence of 0.9 g/l NH 4Cl (e.g., 40% and 66%, respectively, in cells incubated for 95 h), while ammonium assimilation occurred via the glutamate dehydrogenase reaction. Furthermore, growth limitation occurred even in the presence of 0.5 g/l NH 4Cl, when the DO concentration decreased from 135 to 30 μM. The activities of both CS and succinate dehydrogenase were dramatically decreased in cells grown at the lower DO concentration (e.g., 90% and 93% respectively, in a 95 h incubation), while ammonium assimilation was limited due to the low activities of both glutamate dehydrogenase and glutamate synthase. It is concluded that the threshold of ammonium concentration at which growth of A. lipoferum is limited, depends on the DO concentration in the medium. |
en |
| heal.journalName |
Applied Microbiology and Biotechnology |
en |
| dc.identifier.issue |
5-6 |
en |
| dc.identifier.volume |
62 |
en |
| dc.identifier.doi |
10.1007/s00253-003-1349-9 |
en |
| dc.identifier.spage |
574 |
en |
| dc.identifier.epage |
578 |
en |