Growth and energy generation by Enterococcus faecium FAIR-E 198 during citrate metabolism

dc.contributor.author	Sarantinopoulos, P	en
dc.contributor.author	Makras, L	en
dc.contributor.author	Vaningelgem, F	en
dc.contributor.author	Kalantzopoulos, G	en
dc.contributor.author	De Vuyst, L	en
dc.contributor.author	Tsakalidou, E	en
dc.date.accessioned	2014-06-06T06:45:32Z
dc.date.available	2014-06-06T06:45:32Z
dc.date.issued	2003	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/S0168-1605(02)00421-X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2488
dc.subject	Citrate	en
dc.subject	Energy generation	en
dc.subject	Enterococcus faecium	en
dc.subject	Glucose	en
dc.subject	Metabolism	en
dc.subject.other	acetoin	en
dc.subject.other	alcohol	en
dc.subject.other	citric acid	en
dc.subject.other	formic acid	en
dc.subject.other	glucose	en
dc.subject.other	lactic acid	en
dc.subject.other	oxygen	en
dc.subject.other	aerobic capacity	en
dc.subject.other	anaerobic capacity	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial metabolism	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterium isolation	en
dc.subject.other	carbon source	en
dc.subject.other	cheese	en
dc.subject.other	citric acid cycle	en
dc.subject.other	culture medium	en
dc.subject.other	energy yield	en
dc.subject.other	Enterococcus faecium	en
dc.subject.other	glucose metabolism	en
dc.subject.other	glucose utilization	en
dc.subject.other	growth rate	en
dc.subject.other	intermethod comparison	en
dc.subject.other	microbial biomass	en
dc.subject.other	nonhuman	en
dc.subject.other	stoichiometry	en
dc.subject.other	Cheese	en
dc.subject.other	Citric Acid	en
dc.subject.other	Culture Media	en
dc.subject.other	Enterococcus faecium	en
dc.subject.other	Food Microbiology	en
dc.subject.other	Glucose	en
dc.subject.other	Kinetics	en
dc.subject.other	Oxygen	en
dc.subject.other	Enterococcus	en
dc.subject.other	Enterococcus faecium	en
dc.title	Growth and energy generation by Enterococcus faecium FAIR-E 198 during citrate metabolism	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0168-1605(02)00421-X	en
heal.publicationDate	2003	en
heal.abstract	Citrate metabolism by Enterococcus faecium FAIR-E 198, isolated from Greek Feta cheese, was studied in various growth media containing citrate either in the presence of glucose, or as the sole carbon source, both under aerobic and anaerobic conditions. In de Man-Rogosa-Sharpe (MRS) broth with increasing citrate concentrations, cometabolism of citrate and glucose took place. Glucose was stoichiometrically converted into lactate, while citrate into acetate. Glucose consumption and biomass yield were enhanced with increasing initial citrate concentrations, even though maximum specific growth rate was not. When citrate was used as the sole carbon source in increasing initial concentrations, the main end product was acetate. Small amounts of lactate, formate, ethanol, and acetoin were also produced. In all cases, no significant differences were observed between aerobic and anaerobic conditions. However, when citrate was used as sole carbon source, formate production was favored in the absence of oxygen. The present work shows that E. faecium is able to utilize citrate in synthetic media, either in the presence of glucose or as the sole carbon source, resulting in energy production and the formation of aroma compounds. © 2002 Elsevier Science B.V. All rights reserved.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	2	en
dc.identifier.volume	84	en
dc.identifier.doi	10.1016/S0168-1605(02)00421-X	en
dc.identifier.spage	197	en
dc.identifier.epage	206	en