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Genetic diversity among accessions of an ancient olive variety of Cyprus

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dc.contributor.advisor Hatzopoulos, Polydefkis en
dc.contributor.advisor Χατζόπουλος, Πολυδεύκης el
dc.contributor.author Minas, John en
dc.contributor.author Gregoriou, Costas en
dc.contributor.author Demoliou, Cathrine en
dc.contributor.author Kourti, Anna en
dc.contributor.author Κούρτη, Άννα el
dc.contributor.author Μηνάς, Ιωάννης el
dc.contributor.author Γρηγορίου, Κώστας el
dc.contributor.author Δεμολιού, Κατερίνα el
dc.contributor.author Banilas, Georgios en
dc.contributor.author Μπανίλας, Γεώργιος en
dc.date.accessioned 2014-06-06T06:45:31Z
dc.date.available 2014-06-06T06:45:31Z
dc.date.issued 2003-04-08 en
dc.identifier.issn 08312796 en
dc.identifier.uri http://dx.doi.org/10.1139/g03-011 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/2485
dc.title Genetic diversity among accessions of an ancient olive variety of Cyprus en
heal.type journalArticle en
heal.keyword DNA polymorphism en
heal.keyword Germplasm variability en
heal.keyword Olea europaea en
heal.keyword RAPD markers en
heal.keyword DNA Primers en
heal.keyword Random Amplified Polymorphic DNA Technique en
heal.keyword Species specificity en
heal.keyword Variation en
heal.keyword Embryophyta en
heal.identifier.primary 10.1139/g03-011 en
heal.recordProvider Γεωπονικό Πανεπιστήμιο Αθηνών/Τμήμα Γεωπονικής Βιοτεχνολογίας el
heal.recordProvider Ινστιτούτο Γεωπονικών Ερευνών Κύπρου el
heal.recordProvider Cyprus Institute of Neurology and Genetics en
heal.publicationDate 2003-04-08 en
heal.bibliographicCitation Hatzopoulos, Polydefkis. Genetic diversity among accessions of an ancient olive variety of Cyprus, Genome, vol. 46 (3), pp. 370-376, Canadian Science Publishing, 2003 en
heal.abstract To evaluate germplasm variability and to discriminate between accessions of 'Ladolia', an ancient olive variety of Cyprus, different accessions from a germplasm collection were screened with 11 selected oligonucleotide primers in RAPD-PCRs. A total of 49 polymorphic markers were scored, the combination of which resulted in 70 distinct electrophoretic patterns. Based on either unique or combined patterns, all accessions were identified. Seven genotype-specific markers were detected. One RAPD marker could distinguish accessions according to fruit size. Genetic similarities between accessions, estimated using the Dice similarity coefficient, indicated a high degree of genetic diversity among 'Ladolia' accessions. Genetic relationships were estimated by the unweighted pair-group method with arithmetic averaging (UPGMA) and principal components analysis (PCA). Three main groups of accessions were detected. The first group was generally composed of accessions with small-sized fruits and could be further divided into two sub-groups. According to PCA, most accessions with medium- or large-sized fruits were clustered together. Our results support previous observations suggesting that 'Ladolia' is actually a highly variable mixture of genetically distinct landraces. en
heal.publisher Canadian Science Publishing (NRC) en
heal.journalName Genome en
dc.identifier.doi 10.1139/g03-011 en


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