Flow injection analysis system for L-lysine estimation in foodstuffs using a biosensor based on lysine oxidase immobilization on a gold-poly(m-phenylenediamine) electrode

dc.contributor.author	Divritsioti, MH	en
dc.contributor.author	Karalemas, ID	en
dc.contributor.author	Georgiou, CA	en
dc.contributor.author	Papastathopoulos, DS	en
dc.date.accessioned	2014-06-06T06:45:31Z
dc.date.available	2014-06-06T06:45:31Z
dc.date.issued	2003	en
dc.identifier.issn	00032719	en
dc.identifier.uri	http://dx.doi.org/10.1081/AL-120023623	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2481
dc.subject	Electropolymerization	en
dc.subject	Flow injection analysis	en
dc.subject	Lysine biosensor	en
dc.subject	Lysine oxidase immobilization	en
dc.subject	m-phenylenediamine	en
dc.subject.other	1,3 phenylenediamine	en
dc.subject.other	amino acid	en
dc.subject.other	arginine	en
dc.subject.other	cysteine	en
dc.subject.other	glutaraldehyde	en
dc.subject.other	gold	en
dc.subject.other	histidine	en
dc.subject.other	hydrogen peroxide	en
dc.subject.other	lysine	en
dc.subject.other	lysine oxidase	en
dc.subject.other	ornithine	en
dc.subject.other	phenylalanine	en
dc.subject.other	amino acid analysis	en
dc.subject.other	amperometric biosensor	en
dc.subject.other	amperometry	en
dc.subject.other	analytic method	en
dc.subject.other	article	en
dc.subject.other	biosensor	en
dc.subject.other	electrode	en
dc.subject.other	enzyme immobilization	en
dc.subject.other	flow injection analysis	en
dc.subject.other	food analysis	en
dc.subject.other	monitoring	en
dc.subject.other	oxidation	en
dc.subject.other	polymerization	en
dc.subject.other	reproducibility	en
dc.subject.other	Bovinae	en
dc.subject.other	Triticum aestivum	en
dc.title	Flow injection analysis system for L-lysine estimation in foodstuffs using a biosensor based on lysine oxidase immobilization on a gold-poly(m-phenylenediamine) electrode	en
heal.type	journalArticle	en
heal.identifier.primary	10.1081/AL-120023623	en
heal.publicationDate	2003	en
heal.abstract	The development and the evaluation of an amperometric FIA biosensing system for L-lysine determination in foodstuffs is described. The system employs a home-made flow-cell with an amperometric biosensor, prepared by immobilization of lysine oxidase on a gold-poly(m-phenylenediamine) electrode. A peristaltic pump is connected to the flow-cell via a 2-position 6-port sample injector/switching valve for the on-line transfer of the samples. The analytical procedure is based on the oxidation of L-lysine by L-lysine α-oxidase. The produced H2O2 is monitored amperometrically at 650mV in FIA set-up. The lysine biosensor construction procedure comprises: a) the electropolymerization of m-phenylenediamine on a Si-gold strip electrode and b) the immobilization of L-lysine α-oxidase on the poly(m-phenylenediamine) membrane with glutaraldehyde in the presence of bovine serum albumin. Parameters, such as, flow rate, enzyme amount, monomer concentration, matrix effect, effect of interference's, etc., were studied. The flow-cell biosensor system provides a linear response from 1 × 10-3 to 5 × 10-5 M of lysine, with excellent characteristics of reproducibility, stability, and life-time. The system was further applied and evaluated for the estimation of lysine in various hydrolysate food samples (e.g., pasta, milk, wheat flour, etc.) without any pretreatment with an average recovery of 97.3%.	en
heal.journalName	Analytical Letters	en
dc.identifier.issue	9	en
dc.identifier.volume	36	en
dc.identifier.doi	10.1081/AL-120023623	en
dc.identifier.spage	1939	en
dc.identifier.epage	1963	en