Discrimination of the closely related biocontrol agents Macrolophus melanotoma (Hemiptera: Miridae) and M. pygmaeus using mitochondrial DNA analysis

dc.contributor.author	Perdikis, DC	en
dc.contributor.author	Margaritopoulos, JT	en
dc.contributor.author	Stamatis, C	en
dc.contributor.author	Mamuris, Z	en
dc.contributor.author	Lykouressis, DP	en
dc.contributor.author	Tsitsipis, JA	en
dc.contributor.author	Pekas, A	en
dc.date.accessioned	2014-06-06T06:45:29Z
dc.date.available	2014-06-06T06:45:29Z
dc.date.issued	2003	en
dc.identifier.issn	00074853	en
dc.identifier.uri	http://dx.doi.org/10.1079/BER2003265	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2461
dc.subject.other	mitochondrial DNA	en
dc.subject.other	biocontrol agent	en
dc.subject.other	DNA	en
dc.subject.other	genetic analysis	en
dc.subject.other	predator	en
dc.subject.other	animal	en
dc.subject.other	article	en
dc.subject.other	biological pest control	en
dc.subject.other	classification	en
dc.subject.other	female	en
dc.subject.other	genetics	en
dc.subject.other	Hemiptera	en
dc.subject.other	histology	en
dc.subject.other	male	en
dc.subject.other	phylogeny	en
dc.subject.other	reproduction	en
dc.subject.other	season	en
dc.subject.other	species difference	en
dc.subject.other	Animals	en
dc.subject.other	DNA, Mitochondrial	en
dc.subject.other	Female	en
dc.subject.other	Hemiptera	en
dc.subject.other	Male	en
dc.subject.other	Pest Control, Biological	en
dc.subject.other	Phylogeny	en
dc.subject.other	Reproduction	en
dc.subject.other	Seasons	en
dc.subject.other	Species Specificity	en
dc.subject.other	Animalia	en
dc.subject.other	Asteraceae	en
dc.subject.other	Dittrichia	en
dc.subject.other	Dittrichia viscosa	en
dc.subject.other	Hemiptera	en
dc.subject.other	Insecta	en
dc.subject.other	Macrolophus	en
dc.subject.other	Miridae	en
dc.title	Discrimination of the closely related biocontrol agents Macrolophus melanotoma (Hemiptera: Miridae) and M. pygmaeus using mitochondrial DNA analysis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1079/BER2003265	en
heal.publicationDate	2003	en
heal.abstract	The separation of the closely related predatory species Macrolophus melanotoma Costa (= M. caliginosus Wagner) and Macrolophus pygmaeus (Rambur) based exclusively on the different colour pattern of the first antennal segment (white central band in M. melanotoma and entirely black in M. pygmaeus) is rather precarious and their taxonomic status is still in doubt. In the present study their taxonomic status was evaluated by DNA confirmatory analysis and hybridization experiments between M. pygmaeus and a Macrolophus taxon, resembling M. melanotoma, with a first antennal segment entirely black or with a white central band collected from Dittrichia viscosa (L.) W. Greuter (Asteraceae) in southern Greece. Adult females from Dittrichia plants hybridized with males of M. pygmaeus and vice versa did not produce viable eggs. The Macrolophus species from Dittrichia irrespective of the first antennal segment coloration differed from M. pygmaeus in digestive patterns generated by AseI, XbaI, and MseI on 16S rRNA and in RAPD profiles produced by the primers OPA-18 and OPA-20. These results demonstrate that on Dittrichia plants there is a distinct dimorphic taxon, M. melanotoma, as it is the only species of the genus Macrolophus bearing a first antennal segment with a central white band. Given the limitation of the coloration pattern, the mtDNA genetic markers are the appropriate method for the identification of M. melanotoma and M. pygmaeus.	en
heal.journalName	Bulletin of Entomological Research	en
dc.identifier.issue	6	en
dc.identifier.volume	93	en
dc.identifier.doi	10.1079/BER2003265	en
dc.identifier.spage	507	en
dc.identifier.epage	514	en