Developing a scale-up system for the micropropagation of cucumber (Cucumis sativus L.): The effect of growth retardants, liquid culture and vessel size

dc.contributor.author	Konstas, J	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:45:28Z
dc.date.available	2014-06-06T06:45:28Z
dc.date.issued	2003	en
dc.identifier.issn	07217714	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2457
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0037328324&partnerID=40&md5=04ae7e8681338028f84c1ca09f5c44ae	en
dc.subject	Acclimatization	en
dc.subject	Bioreactor	en
dc.subject	Cucumis sativus	en
dc.subject	Flurprimidol	en
dc.subject	Liquid culture	en
dc.subject.other	Antioxidants	en
dc.subject.other	Bioreactors	en
dc.subject.other	Grafts	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Phenols	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Sugars	en
dc.subject.other	Nodal explants	en
dc.subject.other	Vegetable species	en
dc.subject.other	Plant cell culture	en
dc.subject.other	Acclimatization	en
dc.subject.other	Antioxidants	en
dc.subject.other	Ascorbic Acid	en
dc.subject.other	Bioreactors	en
dc.subject.other	Calcium	en
dc.subject.other	Carbohydrate Metabolism	en
dc.subject.other	Cucumis sativus	en
dc.subject.other	Culture Media	en
dc.subject.other	Culture Techniques	en
dc.subject.other	Morphogenesis	en
dc.subject.other	Nitrates	en
dc.subject.other	Phenols	en
dc.subject.other	Phosphates	en
dc.subject.other	Plant Growth Regulators	en
dc.subject.other	Regeneration	en
dc.subject.other	Cucumis	en
dc.subject.other	Cucumis sativus	en
dc.title	Developing a scale-up system for the micropropagation of cucumber (Cucumis sativus L.): The effect of growth retardants, liquid culture and vessel size	en
heal.type	journalArticle	en
heal.publicationDate	2003	en
heal.abstract	We investigated the effect of the growth retardant flurprimidol, the phase of the culture medium (solid versus liquid) and the size of the liquid culture vessel (250-ml flask versus 2.5-1 airlift bioreactor) on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. Flurprimidol at concentrations of 0.1-2 mg l-1 caused considerable growth retardation but increased, albeit slightly, the number of branches and buds and stimulated (solid medium) or reduced (liquid medium) the accumulation of NO3-and PO43-. Flurprimidol had varying effects on the accumulation of soluble sugars and antioxidant compounds. Bioreactor-derived plants showed an increased fresh weight and size but a decreased content of macronutrients, solid sugars, ascorbic acid and free antioxidant phenolics. The majority (95%) of the plants were successfully acclimatized after being graft on squash. The perspective for an efficient, commercial-level use of bioreactors in combination with growth retardants of this commercially important vegetable species is discussed.	en
heal.journalName	Plant Cell Reports	en
dc.identifier.issue	6	en
dc.identifier.volume	21	en
dc.identifier.spage	538	en
dc.identifier.epage	548	en