Design and selection of ligands for affinity chromatography

dc.contributor.author	Labrou, NE	en
dc.date.accessioned	2014-06-06T06:45:28Z
dc.date.available	2014-06-06T06:45:28Z
dc.date.issued	2003	en
dc.identifier.issn	15700232	en
dc.identifier.uri	http://dx.doi.org/10.1016/S1570-0232(03)00098-9	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/2455
dc.subject	Affinity adsorbents	en
dc.subject	Combinatorial design	en
dc.subject	Ligand design	en
dc.subject	Molecular docking	en
dc.subject	Peptides	en
dc.subject	Phage display	en
dc.subject	Proteins	en
dc.subject	Reviews	en
dc.subject	Ribosome display	en
dc.subject	Systematic evolution of ligand by exponential enrichment	en
dc.subject.other	Affinity chromatography	en
dc.subject.other	Molecular structure	en
dc.subject.other	Purification	en
dc.subject.other	Synthesis (chemical)	en
dc.subject.other	Affinity ligands	en
dc.subject.other	Proteins	en
dc.subject.other	carboxypeptidase G2	en
dc.subject.other	dye	en
dc.subject.other	enterotoxin	en
dc.subject.other	formate dehydrogenase	en
dc.subject.other	glucokinase	en
dc.subject.other	interferon	en
dc.subject.other	ligand	en
dc.subject.other	monoclonal antibody	en
dc.subject.other	nucleic acid	en
dc.subject.other	peptide	en
dc.subject.other	phosphotransferase	en
dc.subject.other	synthetic peptide	en
dc.subject.other	trypsin inhibitor	en
dc.subject.other	accuracy	en
dc.subject.other	affinity chromatography	en
dc.subject.other	analytic method	en
dc.subject.other	combinatorial chemistry	en
dc.subject.other	economic aspect	en
dc.subject.other	genetic selection	en
dc.subject.other	mathematical computing	en
dc.subject.other	molecular model	en
dc.subject.other	phage display	en
dc.subject.other	priority journal	en
dc.subject.other	process design	en
dc.subject.other	protein function	en
dc.subject.other	protein purification	en
dc.subject.other	protein structure	en
dc.subject.other	protein targeting	en
dc.subject.other	review	en
dc.subject.other	scale up	en
dc.subject.other	synthesis	en
dc.subject.other	validation process	en
dc.subject.other	Chromatography, Affinity	en
dc.subject.other	Combinatorial Chemistry Techniques	en
dc.subject.other	Ligands	en
dc.title	Design and selection of ligands for affinity chromatography	en
heal.type	other	en
heal.identifier.primary	10.1016/S1570-0232(03)00098-9	en
heal.publicationDate	2003	en
heal.abstract	Affinity chromatography is potentially the most selective method for protein purification. The technique has the purification power to eliminate steps, increase yields and thereby improve process economics. However, it suffers from problems regarding ligand stability and cost. Some of the most recent advances in this area have explored the power of rational and combinatorial approaches for designing highly selective and stable synthetic affinity ligands. Rational molecular design techniques, which are based on the ability to combine knowledge of protein structures with defined chemical synthesis and advanced computational tools, have made rational ligand design feasible and faster. Combinatorial approaches based on peptide and nucleic acid libraries have permitted the rapid synthesis of new synthetic affinity ligands of potential use in affinity chromatography. The versatility of these approaches suggests that, in the near future, they will become the dominant methods for designing and selection of novel affinity ligands with scale-up potential. © 2003 Elsevier Science B.V. All rights reserved.	en
heal.journalName	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences	en
dc.identifier.issue	1-2	en
dc.identifier.volume	790	en
dc.identifier.doi	10.1016/S1570-0232(03)00098-9	en
dc.identifier.spage	67	en
dc.identifier.epage	78	en